中国急救医学
中國急救醫學
중국급구의학
CHINESE JOURNAL OF CRITICAL CARE MEDICINE
2010年
2期
147-150,封3
,共5页
李燕华%李吕力%王铁建%董艳玲
李燕華%李呂力%王鐵建%董豔玲
리연화%리려력%왕철건%동염령
星形胶质细胞%水通道蛋白-9%高渗培养液
星形膠質細胞%水通道蛋白-9%高滲培養液
성형효질세포%수통도단백-9%고삼배양액
Astrocyte%Aquaporin - 9%Hypertonic medium
目的 研究星形胶质细胞水通道蛋白-9(aquaporin-9,AQP9)在高渗培养液中的表达变化及AQP9在高渗性脱水中的作用.方法 取生后2 d的新生Wistar大鼠大脑皮层进行星形胶质细胞纯培养,分别予不同程度的高渗堵养液作用于细胞,建立星形胶质细胞对高渗液反应的实验模型.通过免疫细胞化学和PT-PCR方法研究星形胶质细胞对高渗液的反应及AQP9mRNA和蛋白的表达变化.结果 星形胶质细胞经渗透压为320、333 mmol/L的培养液作用3、6、12、24 h后,AQP9 mRNA和蛋白的表达均显著高于对照组(P<0.05),尤以作用6 h后较明显,而且AQP9 mRNA和蛋白的表达呈显著正相关(r>0.7971,P<0.01).结论 高渗引起AQP9基因表达上调,AQP9在高渗性脱水的早期可能起到重要的代偿作用.
目的 研究星形膠質細胞水通道蛋白-9(aquaporin-9,AQP9)在高滲培養液中的錶達變化及AQP9在高滲性脫水中的作用.方法 取生後2 d的新生Wistar大鼠大腦皮層進行星形膠質細胞純培養,分彆予不同程度的高滲堵養液作用于細胞,建立星形膠質細胞對高滲液反應的實驗模型.通過免疫細胞化學和PT-PCR方法研究星形膠質細胞對高滲液的反應及AQP9mRNA和蛋白的錶達變化.結果 星形膠質細胞經滲透壓為320、333 mmol/L的培養液作用3、6、12、24 h後,AQP9 mRNA和蛋白的錶達均顯著高于對照組(P<0.05),尤以作用6 h後較明顯,而且AQP9 mRNA和蛋白的錶達呈顯著正相關(r>0.7971,P<0.01).結論 高滲引起AQP9基因錶達上調,AQP9在高滲性脫水的早期可能起到重要的代償作用.
목적 연구성형효질세포수통도단백-9(aquaporin-9,AQP9)재고삼배양액중적표체변화급AQP9재고삼성탈수중적작용.방법 취생후2 d적신생Wistar대서대뇌피층진행성형효질세포순배양,분별여불동정도적고삼도양액작용우세포,건립성형효질세포대고삼액반응적실험모형.통과면역세포화학화PT-PCR방법연구성형효질세포대고삼액적반응급AQP9mRNA화단백적표체변화.결과 성형효질세포경삼투압위320、333 mmol/L적배양액작용3、6、12、24 h후,AQP9 mRNA화단백적표체균현저고우대조조(P<0.05),우이작용6 h후교명현,이차AQP9 mRNA화단백적표체정현저정상관(r>0.7971,P<0.01).결론 고삼인기AQP9기인표체상조,AQP9재고삼성탈수적조기가능기도중요적대상작용.
Objective To study the expression of aquaporin - 9 (AQPg) and the role in cultured rat astrocytes after exposured to hypertonic medium.Methods Rat cerebral cortical astrocytes from 2 days newborn Wistar rat were undergone the pure culture and its model of cell dehydration was established by exposed to hypertonic medium.The reactivity and effect on expression of AQP9 mRNA and protein of astrocytes to hypertonic medium were studied by RT - PCR, immunocytochemistry, and imaging analysis.Results Immunocytochemistry and RT - PCR analysis confirmed that the expression of AQP9 protein and mRNA was markedly increased than those in the control group (P < 0.05) after astrocytes cultured for 3, 6, 12, 24 h with hypertonic medium(320, 333 retool/L), especially expression of AQP9 was significantly increased with hypertonic medium for 6 hours.The AQP9 mRNA expression showed a positive relationship with the AQP9 protein expression when astrocytes were cultured with hypertonic medium for 1 day (r>0.7971,P <0.01).Conclusion The expression of AQP9 mRNA and protein increased in hypertonic medium, which indicated that the up - graduated expression of AQP9 may play an important compensation in the early stage of the hypertonic dehydration.
