中国法医学杂志
中國法醫學雜誌
중국법의학잡지
CHINESE JOURNAL OF FORENSIC MEDICINE
2010年
2期
91-93
,共3页
法医毒物分析%毛细管电泳%高频电导检测%γ-羟基丁酸
法醫毒物分析%毛細管電泳%高頻電導檢測%γ-羥基丁痠
법의독물분석%모세관전영%고빈전도검측%γ-간기정산
forensic toxicology analysis%capillary electrophoresis%high frequency conductivity detec-tion%γ-hydroxybutyrate
目的 建立饮料中γ-羟基丁酸(GHB)的毛细管电泳高频电导法检测分析方法 .方法 样品经稀释后直接进样,采用反向分离模式,以0.5mmol/L Na_3PO_4+1.5mmol/LNa_2HPO_4+0.1mmol/L CTAB为电泳分离缓冲介质,分离电压为16kV,进样时间为20s进行电泳分离及高频电导检测.结果 该实验条件下,6.5min内可实现样品快速分析.GHB在10.0～150μ/mL范围内线性关系良好,r=0.990,检出限为3.0μg/mL(S/N=3).选择的3种饮料样本不同添加浓度日间和日内RSD均小于6%,同收率均在95%以上.结论 采用本文方法 ,样品处理简单,方法 快速、灵敏,操作方便,可作为饮料中GHB的一种快速筛选法在相关检测中选用.
目的 建立飲料中γ-羥基丁痠(GHB)的毛細管電泳高頻電導法檢測分析方法 .方法 樣品經稀釋後直接進樣,採用反嚮分離模式,以0.5mmol/L Na_3PO_4+1.5mmol/LNa_2HPO_4+0.1mmol/L CTAB為電泳分離緩遲介質,分離電壓為16kV,進樣時間為20s進行電泳分離及高頻電導檢測.結果 該實驗條件下,6.5min內可實現樣品快速分析.GHB在10.0～150μ/mL範圍內線性關繫良好,r=0.990,檢齣限為3.0μg/mL(S/N=3).選擇的3種飲料樣本不同添加濃度日間和日內RSD均小于6%,同收率均在95%以上.結論 採用本文方法 ,樣品處理簡單,方法 快速、靈敏,操作方便,可作為飲料中GHB的一種快速篩選法在相關檢測中選用.
목적 건립음료중γ-간기정산(GHB)적모세관전영고빈전도법검측분석방법 .방법 양품경희석후직접진양,채용반향분리모식,이0.5mmol/L Na_3PO_4+1.5mmol/LNa_2HPO_4+0.1mmol/L CTAB위전영분리완충개질,분리전압위16kV,진양시간위20s진행전영분리급고빈전도검측.결과 해실험조건하,6.5min내가실현양품쾌속분석.GHB재10.0～150μ/mL범위내선성관계량호,r=0.990,검출한위3.0μg/mL(S/N=3).선택적3충음료양본불동첨가농도일간화일내RSD균소우6%,동수솔균재95%이상.결론 채용본문방법 ,양품처리간단,방법 쾌속、령민,조작방편,가작위음료중GHB적일충쾌속사선법재상관검측중선용.
Objective To establish a fast screening method based on capillary electrophoresis with high frequency conductivity detection for the determination of GHB in beverages. Methods An electrolyte consis-ting of0.5mmol/L Na_3>PO_4, 1.5mmol/LNa_2HPO_4and 0.1mmol/L CTAB was applied to separate the drink-ing samples after they had been simply diluted with water, and the seperation was carried out at voltage of 16kV using CTAB to reverse electroosmotic flow. Results With the optimized condition, the analyte could be baseline separated from interferents in less than 6. 5minutes. The correlation coefficient of GHB was 0. 990 in the range of 10. 0 ～ 150μg/mL, and the limit of detection (LOD) was 3μg/mL (S/N≥3). The coefficients of variation for both intra-day and inter-day were less than 6% and the recoveries were better than 95% at va-rious spiked levels for the beverages. Conclusion The developed electrophoretic analysis shows simple, effec-tive, economic and sensitive, it provides a rapid screening method for GHB determination in application of toxicological analysis.
