CAJ | 학술논문

目的研究人单核细胞源性巨噬细胞向泡沫细胞分化过程中MaxiK通道α-亚单位的表达.方法采用密度梯度离心法从男性健康志愿者外周血中分离单核细胞,经培养后分化为巨噬细胞,并通过加氧化型低密度脂蛋白(OxLDL),建立人巨噬细胞源性泡沫细胞模型,采用RT-PCR、蛋白质印迹及免疫细胞化学方法研究MaxiK 通道α-亚单位的表达.结果巨噬细胞同30 mg/L OxLDL孵育60 h后,细胞内总胆固醇(TC),游离胆固醇(FC)及胆固醇酯(CE)显著增加,并且CE/TC从(14.437±6.781)%提高到(57.946±3.507)%.同时,MaxiK通道α-亚单位表达被下调,但同巨噬细胞相比差异无统计学意义(P>0.05).结论人单核细胞源性巨噬细胞同30 mg/L OxLDL孵育60 h后可分化为泡沫细胞,但MaxiK 通道α-亚单位的表达无明显改变.
목적연구인단핵세포원성거서세포향포말세포분화과정중MaxiK통도α-아단위적표체.방법채용밀도제도리심법종남성건강지원자외주혈중분리단핵세포,경배양후분화위거서세포,병통과가양화형저밀도지단백(OxLDL),건립인거서세포원성포말세포모형,채용RT-PCR、단백질인적급면역세포화학방법연구MaxiK 통도α-아단위적표체.결과거서세포동30 mg/L OxLDL부육60 h후,세포내총담고순(TC),유리담고순(FC)급담고순지(CE)현저증가,병차CE/TC종(14.437±6.781)%제고도(57.946±3.507)%.동시,MaxiK통도α-아단위표체피하조,단동거서세포상비차이무통계학의의(P>0.05).결론인단핵세포원성거서세포동30 mg/L OxLDL부육60 h후가분화위포말세포,단MaxiK 통도α-아단위적표체무명현개변.
Objective To investigate the expression of MaxiK channel α-subunit during human monocyte-derived macrophages differentiating into foam cells. Methods Human peripheral blood monocytes were isolated from male healthy volunteers by density gradient centrifugation, which, by culture, differentiated further into macrophages as a homogeneous monocyte population. The foam cell model originated from human macrophage was established by incubating macrophages with oxidized low density lipoprotein (OxLDL). The expression of MaxiK channel α-subunit was investigated by RT-PCR techniques, Western blotting and immunocytochemistry. Results After incubating macrophages with 30 mg/L OxLDL for 60 hours, the cellular contents of total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) were markedly increased and the ratio of CE/TC was further raised from (14.437±6.781)% to (57.946±3.507) %. Although the expression of MaxiK channel α-subunit was down-regulated during human monocyte-derived macrophages differentiating into foam cells, there was no significant difference between macrophages and foam cells (P>0.05). Conclusion That 30 mg/L OxLDL can lead the monocyte-derived macrophage cultured for 60 hours to differentiate into foam cell, but the expression of MaxiK channel α-subunit does not change obviously.