中成药
中成藥
중성약
CHINESE TRADITIONAL PATENT MEDICINE
2010年
2期
196-199
,共4页
贾晓斌%邵振中%陈彦%袁海建%金晓勇
賈曉斌%邵振中%陳彥%袁海建%金曉勇
가효빈%소진중%진언%원해건%금효용
三草方%提取物%肺腺癌%A549细胞株
三草方%提取物%肺腺癌%A549細胞株
삼초방%제취물%폐선암%A549세포주
Sancao prescription%extract%adenocarcinoma of lung%A549 cells strain
目的:研究三草方(夏枯草、白花蛇舌草、仙鹤草)的不同极性提取部位及其配伍后对人肺腺癌A549细胞株增殖活性的影响.方法:采用醇提取法和水提取法分别制备三草方的95%、60%、30%醇提部位、水提部位;以MTT法测定水煎液和各不同极性部位及其配伍后对A549细胞的增殖活性的影响.结果:在三草方60%醇提部位对A549细胞的IC_(50)最低;60%和95%醇提部位配伍后有明显的协同抑瘤活性.结论:三草方的95%和60%醇提部位间的配伍具有良好的抗肺癌作用.
目的:研究三草方(夏枯草、白花蛇舌草、仙鶴草)的不同極性提取部位及其配伍後對人肺腺癌A549細胞株增殖活性的影響.方法:採用醇提取法和水提取法分彆製備三草方的95%、60%、30%醇提部位、水提部位;以MTT法測定水煎液和各不同極性部位及其配伍後對A549細胞的增殖活性的影響.結果:在三草方60%醇提部位對A549細胞的IC_(50)最低;60%和95%醇提部位配伍後有明顯的協同抑瘤活性.結論:三草方的95%和60%醇提部位間的配伍具有良好的抗肺癌作用.
목적:연구삼초방(하고초、백화사설초、선학초)적불동겁성제취부위급기배오후대인폐선암A549세포주증식활성적영향.방법:채용순제취법화수제취법분별제비삼초방적95%、60%、30%순제부위、수제부위;이MTT법측정수전액화각불동겁성부위급기배오후대A549세포적증식활성적영향.결과:재삼초방60%순제부위대A549세포적IC_(50)최저;60%화95%순제부위배오후유명현적협동억류활성.결론:삼초방적95%화60%순제부위간적배오구유량호적항폐암작용.
AIM: To investigate the ethanolic and aqueous extracts from Sancao prescription (Spica prunellae, Oldenlandia diffuse (willd) Roxb, Herba agrimoniae) on the proliferation of human lung adenocarcinoma cell line (A549). METHODS: 95% ,60% and 30% ethanolic extract and aqueous extract were prepared from Sancao pre-scription. The MTT assay was used to determine the inhibitory action against the proliferation of A549. RESULTS: IC_(50) of 60% ethanolic extract over A549 was one of the lowest in extracts. Combination of 60% and 90% ethanolic extract showed the synergistic antitumour activity. CONCLUSION: Ethanolic extract of Sancao prescription has and effect on human hung adenocarcinoma(A549).
