CAJ | 학술논문

目的体外研究FAP-1在5-氮胞苷(5-azacytidine,5-azaC)诱导的HSG(human salivary gland,HSG,人涎腺导管细胞,起源于人颌下腺闰管细胞)细胞凋亡中的作用,为应用5-azaC对涎腺肿瘤进行治疗奠定理论和实验基础.方法 HSG细胞转染FAP-1反义寡核苷酸前后分别用5-azaC处理,观察转染前后FAP-1表达的变化,同时观察5-azaC对转染前后细胞凋亡作用是否发生变化.结果在形态上转染后的细胞与转染前无明显区别,转染FAP-1反义寡核苷酸链的HSG细胞FAP-1表达较转染前减弱,转染后HSG细胞对5-azaC比转染前敏感,加入5umol/L 5-azaC后,凋亡细胞数增加.结论 FAP-1在5-azaC诱导的HSG细胞凋亡中起一定作用.
목적 체외연구FAP-1재5-담포감(5-azacytidine,5-azaC)유도적HSG(human salivary gland,HSG,인연선도관세포,기원우인합하선윤관세포)세포조망중적작용,위응용5-azaC대연선종류진행치료전정이론화실험기출.방법 HSG세포전염FAP-1반의과핵감산전후분별용5-azaC처리,관찰전염전후FAP-1표체적변화,동시관찰5-azaC대전염전후세포조망작용시부발생변화.결과 재형태상전염후적세포여전염전무명현구별,전염FAP-1반의과핵감산련적HSG세포FAP-1표체교전염전감약,전염후HSG세포대5-azaC비전염전민감,가입5umol/L 5-azaC후,조망세포수증가.결론 FAP-1재5-azaC유도적HSG세포조망중기일정작용.
Objective To investigate the role of FAP-1 in apoptosis of human salivary gland cells (HSG) inducted by 5-azacytidine (5-azaC) .Methods HSG cells transfected by FAP-1 antisense oligonucleotides were treated with 5-azaC. The morphology of HSG and expression of FAP-1 was then examined.Results The configuration of HSG cells did not change after transfected by FAP-1 antisense oligonucleotides, compared with HSG cells that not transfected by. The expression of FAP-1 was knock-down after transfected by FAP-1 antisense oligonucleotides. More HSG cells underwent apoptosis when 5umol/L 5-azaC was added after transfected by FAP-1 antisense oligonucleotide.Conclusion FAP-1 play an important role in the apoptosis effect of 5-azaC on HSG cell in vitro.