CAJ | 학술논문

目的为了建立一种快速、简便、实用的可用于育龄妇女血清中巨细胞病毒(CMV)-IgG抗体的筛查方法,并初步探索其应用价值. 方法建立重组CMV(pp150+52)抗原;以国家人口和计划生育委员会科学技术研究所(简称本所)建立的重组CMV(pp150+52)抗原作为包被抗原,700 IU/L作为临界值,用间接ELISA方法,对深圳宝安区1 024例血清标本进行CMV-IgG抗体的检测. 结果该方法灵敏度为13 IU/L,批内变异系数4.7%～7.0%,批间变异系数7.6%～14.4%,并与SORIN公司生产的试剂盒进行了比较,2种方法呈最度相关,Kappa值为0.82;深圳宝安区1 024例血清标本CMV-IgG抗体阳性率为93.3%(955/1 024),阴性率为6.7%(69/1 024). 结论本所建立的ELISA方法快速、简便和实用,可用于育龄妇女CMV-IgG抗体的筛查.
목적 위료건립일충쾌속、간편、실용적가용우육령부녀혈청중거세포병독(CMV)-IgG항체적사사방법,병초보탐색기응용개치. 방법 건립중조CMV(pp150+52)항원;이국가인구화계화생육위원회과학기술연구소(간칭본소)건립적중조CMV(pp150+52)항원작위포피항원,700 IU/L작위림계치,용간접ELISA방법,대심수보안구1 024례혈청표본진행CMV-IgG항체적검측. 결과 해방법 령민도위13 IU/L,비내변이계수4.7%～7.0%,비간변이계수7.6%～14.4%,병여SORIN공사생산적시제합진행료비교,2충방법 정최도상관,Kappa치위0.82;심수보안구1 024례혈청표본CMV-IgG항체양성솔위93.3%(955/1 024),음성솔위6.7%(69/1 024). 결론 본소건립적ELISA방법 쾌속、간편화실용,가용우육령부녀CMV-IgG항체적사사.
Objective To establish a rapid, serum and convenient method for the screening of cy-tomegalovirus (CMV) specific IgG antibody in sera samples of women of childbearing age, and to evaluate the preliminary significance of application. Methods The measurement of CMV-IgG was conducted by indirect enzyme linked immunosorbent assay using recombinant CMV-Ag (pp150 + 52) as coating antigen, and 700 IU/L as Cut-off value. Results The specific CMV-IgG antibodies in sera were detected in 1 024 women of childbearing age in Shenzhen, by using the method established by our study and the ELISA assay kid provided by Diasorin Co. The positive rate, negative rate, sensitivity of our method was 93. 3 %, 6.7 %, and 13 IU/L, respectively. The intrasasay coefficient of variation was between 4. 7 % - 7.0 % and the interassay coefficient of variation was between 7.6% - 14. 4 %. The Kappa value between our method and ELISA assay kid provided by Diasofin Co. was 0. 82, indicating a good relativity. Conclusion Our ELISA assay is a rapid, easy and convenient method and could be used for screening the CMV -IgG specific antibodies among premarital women.