CAJ | 학술논문

于花铃期对科棉1号棉株进行连续48 h的不同温度处理,用处理后的叶片饲喂棉铃虫,研究叶片活性、毒蛋白和棉铃虫死亡率之间的关系.结果表明,游离氨基酸含量一直增加,12 h内增加最快;可溶性蛋白含量一直下降,12 h内降幅最大.毒蛋白含量24 h内显著下降,12 h内降幅最大.25 ℃处理下的死亡率明显高于20℃和35℃处理.同一温度下,胁迫时间越长,棉铃虫取食后的死亡率越低.棉铃虫取食72 h后的死亡率明显低于144 h后的死亡率.取食72 h后的死亡率与可溶性蛋白、游离氨基酸和毒蛋白含量呈显著或极显著相关,与可溶性蛋白和毒蛋白变化量之间呈显著相关.
우화령기대과면1호면주진행련속48 h적불동온도처리,용처리후적협편사위면령충,연구협편활성、독단백화면령충사망솔지간적관계.결과표명,유리안기산함량일직증가,12 h내증가최쾌;가용성단백함량일직하강,12 h내강폭최대.독단백함량24 h내현저하강,12 h내강폭최대.25 ℃처리하적사망솔명현고우20℃화35℃처리.동일온도하,협박시간월장,면령충취식후적사망솔월저.면령충취식72 h후적사망솔명현저우144 h후적사망솔.취식72 h후적사망솔여가용성단백、유리안기산화독단백함량정현저혹겁현저상관,여가용성단백화독단백변화량지간정현저상관.
With Bt transgenic cotton plants of Kemian 1 as the testing material, temperature stress experiments (20℃, 25℃, 35℃,and 40℃) were carried out in temperature and light controlled compartments to investigate Bt insecticidal protein content in cotton leaves at flowering stage, the death rate of bollworm fed on cotton leaves, and the relationship between them.The results indicated that free amino acid content increased under temperature stress, with the biggest increment during the first 12 h.The content of soluble protein decreased, presenting a sharp decrement during the first 12 h.Bt insecticidal protein content in leaves subjected to temperature stress decreased significantly during the first 24 h, and the biggest decrements occurred during the first 12 h.The death rate of bollworm fed on the leaves subjected to 25℃ was higher than the death rates of bollworm eating the leaves subjected to 20℃ and 35℃.Under the same temperature, the longer the leaves were subjected to temperature stress, the lower the death rate of bollworm fed on these leaves was.The death rate of bollworm 72 h after eating the leaves was significantly higher than the death rate of bollworm 144 h after eating the leaves.Correlation analyses showed that the contents of Bt insecticidal protein, free amino acid, soluble protein and the death rate of bollworm 72 h after eating the leaves were significantly correlated at 0.05 level or at 0.01 level.The increment of free amino acid, the decrement of Bt insecticidal protein, and the death rate of bollworm 72 h after eating the treated leaves were significantly correlated at 0.05 level.