CAJ | 학술논문

目的建立人脑脊液(cerebrospinal fluid,CSF)中两性霉素B(amphotericin B,AmB)和伊曲康唑(itraconazole,ITZ)含量测定的高效液相色谱方法,并用于隐球菌性脑膜炎患者脑脊液样品的测定.方法脑脊液样品经固相萃取后直接进样.AmB测定采用Gemini 5w C18柱,流动相为5 mmol/L乙二胺四乙酸二钠(醋酸调pH至4.5)/乙腈(62/38,V/V),流速0.3 mL/min,DAD检测器:检测波长410 nm.ITZ测定采用YMC C18柱,流动相为0.2%三氟乙酸/乙腈(45/55,V/V),流速1.1 mL/min,荧光检测器:激发波长260 nm,发射波长365 nm.结果 AmB和ITZ的线性范围均为5 ng/mL～100 ng/mL,线性相关系数大于0.999,最低定量限为5 ng/mL,低、中、高浓度质控样品在日内、日间精密度均小于7.6%.结论该方法灵敏、快速、简便,适用于隐球菌性脑膜炎患者治疗药物监测.
목적 건립인뇌척액(cerebrospinal fluid,CSF)중량성매소B(amphotericin B,AmB)화이곡강서(itraconazole,ITZ)함량측정적고효액상색보방법,병용우은구균성뇌막염환자뇌척액양품적측정.방법 뇌척액양품경고상췌취후직접진양.AmB측정채용Gemini 5w C18주,류동상위5 mmol/L을이알사을산이납(작산조pH지4.5)/을정(62/38,V/V),류속0.3 mL/min,DAD검측기:검측파장410 nm.ITZ측정채용YMC C18주,류동상위0.2%삼불을산/을정(45/55,V/V),류속1.1 mL/min,형광검측기:격발파장260 nm,발사파장365 nm.결과 AmB화ITZ적선성범위균위5 ng/mL～100 ng/mL,선성상관계수대우0.999,최저정량한위5 ng/mL,저、중、고농도질공양품재일내、일간정밀도균소우7.6%.결론 해방법령민、쾌속、간편,괄용우은구균성뇌막염환자치료약물감측.
Objective To establish a sensitive, rapid and simple high-performance liquid chromatography (HPLC) method for the determination of amphotericin B (AraB) and itraconazole (ITZ) in human cerebrospinal fluid (CSF). Methods Solid-phase extraction (SPE) was involved in sample preparation in this method, followed by reverse-phase separation under two different chromatographic systems. Results The tinearity for both AraB and ITZ ranged from 5 ng/mL to 100 ng/mL and the inter-day and intra-day RSD were both below 7.6% at the concentrations of 20,50 and 100 ng/mL. Conclusions The method we established has been applied to the therapeutic drug monitoring on cryptococcal-meningitis-infected patients who were given comedication of these two drugs.