上海师范大学学报(自然科学版)
上海師範大學學報(自然科學版)
상해사범대학학보(자연과학판)
JOURNAL OF SHANGHAI TEACHERS UNIVERSITY(NATURAL SCIENCES)
2008年
1期
74-81
,共8页
2-氨基乙硫醇:尿酸%抗坏血酸%共价修饰
2-氨基乙硫醇:尿痠%抗壞血痠%共價脩飾
2-안기을류순:뇨산%항배혈산%공개수식
2-aminoethanethiol%uric acid%ascorbic acid%covalent modification
采用电化学氧化法将2-氨基乙硫醇共价键合在玻碳电极表面,并采用X-射线光电子能谱和电化学方法对其进行了表征.2-氨基乙硫醇修饰的玻碳电极对尿酸显示出较好的催化氧化作用,表现在尿酸氧化过电位的负移(230 mV)和峰电流的增加.并且,尿酸的氧化峰电流在8.0×10-6～2.0×10-4mol/L内随尿酸浓度的增加而线性增大,相关系数为0.9976,检测限为4.8×10-4mol/L.该法被成功地用于人尿中尿酸含量的测定.另外,该修饰电极可将常规电极上尿酸和抗坏血酸重叠的氧化波进行有效的分离,由此,该法有望用于两者的同时测定.
採用電化學氧化法將2-氨基乙硫醇共價鍵閤在玻碳電極錶麵,併採用X-射線光電子能譜和電化學方法對其進行瞭錶徵.2-氨基乙硫醇脩飾的玻碳電極對尿痠顯示齣較好的催化氧化作用,錶現在尿痠氧化過電位的負移(230 mV)和峰電流的增加.併且,尿痠的氧化峰電流在8.0×10-6～2.0×10-4mol/L內隨尿痠濃度的增加而線性增大,相關繫數為0.9976,檢測限為4.8×10-4mol/L.該法被成功地用于人尿中尿痠含量的測定.另外,該脩飾電極可將常規電極上尿痠和抗壞血痠重疊的氧化波進行有效的分離,由此,該法有望用于兩者的同時測定.
채용전화학양화법장2-안기을류순공개건합재파탄전겁표면,병채용X-사선광전자능보화전화학방법대기진행료표정.2-안기을류순수식적파탄전겁대뇨산현시출교호적최화양화작용,표현재뇨산양화과전위적부이(230 mV)화봉전류적증가.병차,뇨산적양화봉전류재8.0×10-6～2.0×10-4mol/L내수뇨산농도적증가이선성증대,상관계수위0.9976,검측한위4.8×10-4mol/L.해법피성공지용우인뇨중뇨산함량적측정.령외,해수식전겁가장상규전겁상뇨산화항배혈산중첩적양화파진행유효적분리,유차,해법유망용우량자적동시측정.
2-Amincethanethiol (2-AET) was eovalently grafted on glassy carbon electrode (CCE) by amine cation radical formation in the electro-oxidation process of the amino-containing compound. The improved electrochemical behavior of uric acid at the modified electrode showed that the electrode modified with 2-AET exhibited efficient elec-tro-catalytic oxidation for uric acid. The anodic overpotential was reduced about 230 mV compared with that obtained at bare GCE. The enhanced voltammetric response can be used for the determination of uric acid. The linear calibra- tion curve was obtained over the range from 8.0×10-6 mol/L to 2.0×10-4 mol/L with slop (μA/μM) and coefficient correlation of 3.5 and 0.9976, respectively. The detection limit (3σ) for uric acid was 4.8×10-7 mol/L. The relative standard derivation was 2.7% at a concentration of 5.0×10-3 mol/L uric acid. The practical utility of the proposed method was demonstrated by the measurement of uric acid in human urine without any preliminary treatmeut. Moreover, the catalytic effect of 2-AET/GCE to uric acid led to the voltammetric resolution of the overlapping oxidation wave of uric acid and ascorbic acid in a mixture, which allows the simultaneous determination of uric acid and ascorbic acid in a same sample.
