CAJ | 학술논문

目的用体外培养的传代细胞研究三七总皂甙对大鼠肝星状细胞增殖的影响.方法分别将0 mg/ml、0.01mg/ml、0.05mg/ml、0.25mg/ml、1.00mg/ml和1 25 mg/ml浓度的三七总皂甙作用于HSC- T6细胞,用MTT比色法研究三七总皂甙对细胞增殖的影响.结果培养24 h和48 h后,0.25 mg/ml,1.00mg/ml和1 25 mg/mI浓度的三七总皂甙对HSC- T6细胞增殖有明显的抑制作用(P＜0.05);0mg/mI、0.01mg/ml、0.05mg/ml、0.25mg/ml,1.00mg/ml和1.25mg/ml浓度的三七总皂甙对HSC-T6细胞增殖抑制率呈线性关系,并有明显剂量效应关系,经相关回归分析,培养24 h后,Y=0.486402X+0.037362,r=0 982181,P＜0.002;培养48 h后,Y=0.526087.X+0.071327,r=0.992046,P＜0.001.结论三七总皂甙对HSC T6细胞增殖有明显抑制作用.
목적 용체외배양적전대세포연구삼칠총조대대대서간성상세포증식적영향.방법 분별장0 mg/ml、0.01mg/ml、0.05mg/ml、0.25mg/ml、1.00mg/ml화1 25 mg/ml농도적삼칠총조대작용우HSC- T6세포,용MTT비색법연구삼칠총조대대세포증식적영향.결과 배양24 h화48 h후,0.25 mg/ml,1.00mg/ml화1 25 mg/mI농도적삼칠총조대대HSC- T6세포증식유명현적억제작용(P＜0.05);0mg/mI、0.01mg/ml、0.05mg/ml、0.25mg/ml,1.00mg/ml화1.25mg/ml농도적삼칠총조대대HSC-T6세포증식억제솔정선성관계,병유명현제량효응관계,경상관회귀분석,배양24 h후,Y=0.486402X+0.037362,r=0 982181,P＜0.002;배양48 h후,Y=0.526087.X+0.071327,r=0.992046,P＜0.001.결론 삼칠총조대대HSC T6세포증식유명현억제작용.