CAJ | 학술논문

采用生物信息学方法,从随机测序而构建的油茶优良无性系湘林1号种子EST文库中初步鉴定了与茶油脂肪酸形成相关的大部分关键酶基因,并对其中几个直接控制不饱和脂肪酸的形成与转化的基因进行了相应的序列分析.
채용생물신식학방법,종수궤측서이구건적유다우량무성계상림1호충자EST문고중초보감정료여다유지방산형성상관적대부분관건매기인,병대기중궤개직접공제불포화지방산적형성여전화적기인진행료상응적서렬분석.
In order to determine the molecular mechanism of forming high quality tea-oil, the EST library technology was used to find out the main expressed genes controlling the fatty acid biosynthesis in camellia oleifera seeds. A high quality full-length cDNA library was constructed successfully using the nearly matured seeds of elite cultivar Xianglin-1 planted widely in China,then more than two thousand clones were randomly sequenced from the 5' orientation, which was used to construct a high representational EST library. After the bioinformatic analysis of this EST library, 15 main genes controlling the biosynthesis of unsaturated fatty acids in camellia oleifera seeds were obtained.