CAJ | 학술논문

背景博尔纳病病毒(Borna disease virus,BDV)是一种高度嗜神经的RNA病毒,是人畜共患病博尔纳病(Borna disease,BD)的病原体,可引起从鸟到灵长类的多种动物的中枢神经系统感染[1],表现为以中枢神经系统功能障碍为特征的BD.近年研究发现BDV感染与一些神经精神疾病的发病有关,尤其是精神疾病.但有关BDV感染与抑郁症(depressive disorder,DD)发病之间的关系,目前国内外研究尚有争议.本研究从分子生物学角度进一步探讨BDV与DD发病之间的关系.方法采用巢式逆转录酶聚合酶链反应(nRT-PCR)结合荧光定量聚合酶链反应(FQ-PCR)检测了60例DD患者和120名健康人外周血单个核细胞(PBMCs)中BDV p24基因片段,对FQ-PCR阳性产物进行克隆和基因序列测定,测序结果与人和动物来源的BDV分离株以及标准株Strain V和He/80进行序列比较.对两组阳性率进行Fisher精确概率检验.结果 DD组BDV p 24基因片段阳性率为5%(3/60);健康组阳性率为0%(0/120).DD组阳性率高于健康组,差异有显著性意义(P＜0.05).测序结果为5'-CCCTCCAAGTGGAAACCATCCAGACAGCTCAGCGGTGCGACCACTCCGACAGCATCAGGATTCTTGGCGAGAACATCAAGATACTG-3'.登陆美国国家生物技术信息中心,证实所获得目的基因片段确系BDV p 24基因片断,与人类基因组片段和其他病毒基因组片段无同源性.其与马源的BDV病毒株H1766序列比较亲缘关系最近,同源性为97.68%,在2个位点出现突变(nt 1675 T→C,nt 1678 C→T).与其他国际公认的标准病毒株Strain V和He/80比较,同源性分别为96.51%和95.35%,碱基互换中局限于T-C、和A→G两种.结论中国的DD患者中存在BDV感染,重庆地区DD的发病可能与BDV感染有关.
배경 박이납병병독(Borna disease virus,BDV)시일충고도기신경적RNA병독,시인축공환병박이납병(Borna disease,BD)적병원체,가인기종조도령장류적다충동물적중추신경계통감염[1],표현위이중추신경계통공능장애위특정적BD.근년연구발현BDV감염여일사신경정신질병적발병유관,우기시정신질병.단유관BDV감염여억욱증(depressive disorder,DD)발병지간적관계,목전국내외연구상유쟁의.본연구종분자생물학각도진일보탐토BDV여DD발병지간적관계.방법 채용소식역전록매취합매련반응(nRT-PCR)결합형광정량취합매련반응(FQ-PCR)검측료60례DD환자화120명건강인외주혈단개핵세포(PBMCs)중BDV p24기인편단,대FQ-PCR양성산물진행극륭화기인서렬측정,측서결과여인화동물래원적BDV분리주이급표준주Strain V화He/80진행서렬비교.대량조양성솔진행Fisher정학개솔검험.결과 DD조BDV p 24기인편단양성솔위5%(3/60);건강조양성솔위0%(0/120).DD조양성솔고우건강조,차이유현저성의의(P＜0.05).측서결과위5'-CCCTCCAAGTGGAAACCATCCAGACAGCTCAGCGGTGCGACCACTCCGACAGCATCAGGATTCTTGGCGAGAACATCAAGATACTG-3'.등륙미국국가생물기술신식중심,증실소획득목적기인편단학계BDV p 24기인편단,여인류기인조편단화기타병독기인조편단무동원성.기여마원적BDV병독주H1766서렬비교친연관계최근,동원성위97.68%,재2개위점출현돌변(nt 1675 T→C,nt 1678 C→T).여기타국제공인적표준병독주Strain V화He/80비교,동원성분별위96.51%화95.35%,감기호환중국한우T-C、화A→G량충.결론 중국적DD환자중존재BDV감염,중경지구DD적발병가능여BDV감염유관.
Background Evidences, from recent studies, suggested that Borna disease virus (BDV) infection might be associated with human neuropsychosis, especially psychiatric disorders including depressive disorder(DD). However, controversy existed about the association between BDV infection and pathogenesis of DD. This study was to explore further whether the infection of Borna disease virus (BDV) is associated with the pathogenesis of depressive disorder (DD).Methods The p 24 fragment of BDV RNA in peripheral blood mononuclear cells (PBMCs) from 60DD patients and 120 healthy volunteers was detected by nested reverse transcriptase polymerase chain reaction (nRT-PCR) combined with fluorescence quantitative polymerase chain reaction (FQPCR). Positive products were cloned and sequenced before being compared with Strain V and strain He/80, from humans and animals.Results The positive rate (5%, 3/60) of BDV p 24 in PBMCs from the DD patients was significantly higher than that (0%, 0/120) from healthy volunteers ( P＜0. 05). The gene sequence for the positive products showed BDV p 24 in PBMCs from DD patients in Chongqing was most homophylic with H1766 strain detected from iii horses (97.68%), with 2 situs mutations (nt 1675 T→C, nt 1678 C→T), and also similar to the standard strain V(96. 51%)and He/80(95.35 %), with basic exchanges limited to T- C and A→G.Conclusions There was BDV infection in the DD patients in China, which indicated that the pathogenesis of DD in human beings in Chongqing might be associated with the infection of BDV.