CAJ | 학술논문

目的:探讨针灸对单纯性肥胖病瘦素和胰岛素抵抗的作用. 方法:采用 RT-PCR和 Northern Blot技术测定瘦素受体( OB-R)基因表达水平,放射免疫分析测定血清和下丘脑瘦素( Leptin)和胰岛素( INS)的含量.观察针灸治疗前后单纯性肥胖患者血清瘦素、 INS、脂质水平的变化;还观察针刺治疗前后肥胖大鼠体质量、 Lee's指数、体脂、血清和下丘脑瘦素和 INS的含量以及下丘脑 OB-R基因表达的变化. 结果 :单纯性肥胖患者瘦素、 INS、总胆固醇( TC)、三酰甘油( TG)、低密度脂蛋白-胆固醇( LDL-C)的含量均显著高于正常人水平;而高密度脂蛋白-胆固醇( HDL-C)却显著低于正常人水平,针灸治疗后患者瘦素、 INS、 TC、 TG、 LDL-C的含量均明显回降,而 HDL-C含量却明显回升,这种变化与减肥疗效有关.肥胖大鼠体质量、 Lee's指数、体脂及血清瘦素和 INS水平均显著高于正常大鼠,而下丘脑瘦素和 INS水平及 OB-R基因表达水平均明显低于正常大鼠.针刺治疗取得良好减肥疗效的同时,肥胖大鼠血清瘦素和 INS均明显回降,而下丘脑瘦素和 INS水平以及 OB-R基因表达水平却明显升高. 结论 :针灸对肥胖机体中枢和外周瘦素和 INS水平的良性调整作用以及促进下丘脑 OB-R基因表达可能是针灸纠正瘦素和胰岛素抵抗以及异常的内分泌代谢的重要机制.
목적:탐토침구대단순성비반병수소화이도소저항적작용. 방법:채용 RT-PCR화 Northern Blot기술측정수소수체( OB-R)기인표체수평,방사면역분석측정혈청화하구뇌수소( Leptin)화이도소( INS)적함량.관찰침구치료전후단순성비반환자혈청수소、 INS、지질수평적변화;환관찰침자치료전후비반대서체질량、 Lee's지수、체지、혈청화하구뇌수소화 INS적함량이급하구뇌 OB-R기인표체적변화. 결과 :단순성비반환자수소、 INS、총담고순( TC)、삼선감유( TG)、저밀도지단백-담고순( LDL-C)적함량균현저고우정상인수평;이고밀도지단백-담고순( HDL-C)각현저저우정상인수평,침구치료후환자수소、 INS、 TC、 TG、 LDL-C적함량균명현회강,이 HDL-C함량각명현회승,저충변화여감비료효유관.비반대서체질량、 Lee's지수、체지급혈청수소화 INS수평균현저고우정상대서,이하구뇌수소화 INS수평급 OB-R기인표체수평균명현저우정상대서.침자치료취득량호감비료효적동시,비반대서혈청수소화 INS균명현회강,이하구뇌수소화 INS수평이급 OB-R기인표체수평각명현승고. 결론 :침구대비반궤체중추화외주수소화 INS수평적량성조정작용이급촉진하구뇌 OB-R기인표체가능시침구규정수소화이도소저항이급이상적내분비대사적중요궤제.
AIM:To study the function of acupuncture on the leptin and insulin resistance in simple obesity. METHODS:The techniques of RT-PCR and Northern Blot were adopted to test the gene expression of OB-R and the radioimmunoassay was used to test the serum and hypothalamus leptin and insulin in order to observe the changes of serum leptin, insulin and lipide in simple obesity patients and the changes of weight,Lee's index,somatic fat,the contents of serum and hypothalamus leptin and insulin as well as the expression of hypothalamus OB-R gene in obesity rats before and after acupuncture treatment. RESULTS:The levels of leptin,insulin(INS),total cholesterol(TC), triglyceride(TG),and low-density lipoprotein cholesterol(LDL-C) in simple obesity patients were obvious higher than those in the normal group.But the level of high-density lipoprotein-cholesterol(HDL-C) was obviously lower than that of the normal group.After acupuncture treatment,the levels of leptin,INS,TC,TG,and LDL-C of patients were obviously reduced,and the level of HDL-C was obviously increased.This was related to the therapeutic effect of acupuncture. The obesity rats' weight,Lee's index,somatic fat,serum leptin and INS were all obviously higher than those of the normal rats,but the levels of the hypothalamus leptin and insulin and OB-R gene expression were all obviously lower than those of the normal rats.With acupuncture,the weight was lost,at the same time,the levels of the serum leptin and insulin of obesity rats were obviously decreased,while the level of the hypothalamus leptin and insulin and OB-R gene expression were obviously increased. CONCLUSIONS:The function of acupuncture in regulation of the central and peripheral leptin and insulin levels and in promotion of the hypothalamus OB-R gene expression of the obesity patients may be the important mechanism in correcting the leptin and insulin resistance and the abnormal endocrine metabolism.