中国科学C辑(英文版)
中國科學C輯(英文版)
중국과학C집(영문판)
SCIENCE IN CHINA (Series C)
2001年
4期
337-344
,共8页
李乐农%郭宝江%张季平%江涛%常文瑞%梁栋材
李樂農%郭寶江%張季平%江濤%常文瑞%樑棟材
리악농%곽보강%장계평%강도%상문서%량동재
The selenium-containing phycocyanin from the selenium-rich algae (Spirulina platensis) has been crystallized in two crystal forms by the hanging-drop vapor diffusion techniques. A chromatographic procedure of gel filtration and anion exchange was used for purification. Form I crystal with space group P21 and cell parameters a =108.0 ?, b = 117.0 ?, c = 184.0 ?, b = 90.2° and 12(ab) units in the asymmetric unit was obtained by using (NH4)2SO4 as precipitant. These crystals diffract up to 2.8 ?. Form II crystal obtained by using PEG4000 as precipitant belongs to space group P63 with unit cell constants a = 155.0 ?, c = 40.3 ?, g =120.0° and one(ab) unit in the asymmetric unit. The crystals diffract beyond 2.9 ?. The possible stacking forms of phycocyanin molecules in the first crystal form were discussed.
