CAJ | 학술논문

研究应用基因组原位杂交、染色体C-分带和RAPD技术，对八倍体小黑麦×普通小麦杂种F2经电离辐射处理后的高代材料98-60进行了检测。基因组原位杂交结果表明，该材料为小麦-黑麦异代换系。进一步通过C-分带分析表明，该品系为5R代换系，并且还包含有5AS/6AS 小麦种内的染色体易位。通过RAPD分析，在该品系中找到了来源于八倍体小黑麦亲本"新麦73"的黑麦染色体特异扩增产物OPA-01350和与两个亲本不同的特异重组产物OPF-14800、OPF-14920，进一步验证了基因组原位杂交和C-分带的鉴定结果。
연구응용기인조원위잡교、염색체C-분대화RAPD기술，대팔배체소흑맥×보통소맥잡충F2경전리복사처리후적고대재료98-60진행료검측。기인조원위잡교결과표명，해재료위소맥-흑맥이대환계。진일보통과C-분대분석표명，해품계위5R대환계，병차환포함유5AS/6AS 소맥충내적염색체역위。통과RAPD분석，재해품계중조도료래원우팔배체소흑맥친본"신맥73"적흑맥염색체특이확증산물OPA-01350화여량개친본불동적특이중조산물OPF-14800、OPF-14920，진일보험증료기인조원위잡교화C-분대적감정결과。
GISH,chromosome C-banding and RAPD techniques were used in the detection of rye chromatin in high generation offspring (98-60) of radiation treated F2 of crossing between octoploid triticale and common wheat．According to the result of GISH，these offspring are substitution lines，further analysis by C-banding reveal the existence of 5R chromosome and a translocation of 5AS/6AS．These were also confirmed by RAPD that a amplified fragment,OPA-01350，derives from octoploid triticale line "Xinmai 73" and two amplified recombination products,OPF-14800 and OPF-14920,are different from genomic DNA of two parents.