CAJ | 학술논문

目的　观察肺癌组织及癌旁肺组织MDR1-mRNA、MRP-mRNA及LRP-mRNA的表达。方法　采用RT-PCR法检测30例肺癌患者癌组织和正常肺组织中MDR1-mRNA、MRP-mRNA和LRP-mRNA的表达情况。结果　MDR1-mRNA在肺癌组织及癌旁肺组织的表达阳性率分别为40％及16.67％(P＝0.045)，其表达与细胞分化程度、临床分期及病理类型无明显关系。MRP-mRNA在肺癌组织及癌旁肺组织的表达分别为43.33％及26.67％，低分化者MRP的表达率明显高于中高分化者（P＝0.03），其表达与临床分期无明显关系。LRP-mRNA在肺癌组织及癌旁肺组织的表达分别为56.67％及10％（P＝0.000?4），其表达与肿瘤类型、临床分期及癌细胞的分化程度无明显关系。30例肺癌组织中MDR1-mRNA、MRP-mRNA、LRP-mRNA共同表达者7例（23.33％），三者均无表达者10例（33.33％），三者的一致性达56.67％。结论　MDR1-mRNA、MRP-mRNA、LRP-mRNA在肺癌的耐药中可能起重要作用。
목적　관찰폐암조직급암방폐조직MDR1-mRNA、MRP-mRNA급LRP-mRNA적표체。방법　채용RT-PCR법검측30례폐암환자암조직화정상폐조직중MDR1-mRNA、MRP-mRNA화LRP-mRNA적표체정황。결과　MDR1-mRNA재폐암조직급암방폐조직적표체양성솔분별위40％급16.67％(P＝0.045)，기표체여세포분화정도、림상분기급병리류형무명현관계。MRP-mRNA재폐암조직급암방폐조직적표체분별위43.33％급26.67％，저분화자MRP적표체솔명현고우중고분화자（P＝0.03），기표체여림상분기무명현관계。LRP-mRNA재폐암조직급암방폐조직적표체분별위56.67％급10％（P＝0.000?4），기표체여종류류형、림상분기급암세포적분화정도무명현관계。30례폐암조직중MDR1-mRNA、MRP-mRNA、LRP-mRNA공동표체자7례（23.33％），삼자균무표체자10례（33.33％），삼자적일치성체56.67％。결론　MDR1-mRNA、MRP-mRNA、LRP-mRNA재폐암적내약중가능기중요작용。
Objective　To investigate the expression of multidrug resistance （MDR1）, multidrug resistance-associated protein (MRP) and lung resistance protein (LRP) genes in patients with non-small cell lung cancer (NSCLC). Methods　Expression of MDR1, MRP and LRP genes was detected in 30 NSCLC patients by RT-PCR method. Results　The positive rates of MDR1 expression were 40％ and 16.67％ respectively in lung cancer tissues and normal lung tissues (P＝0.045), and it was not associated with the degree of cell differentiation, histological classification and the clinical stage. The positive rates of MRP expression were 43.33％ and 26.67％ respectively in lung cancer tissues and normal lung tissues. Its expression was related to degree of cell differentiation (P＝0.03), but not to the histological classification and the clinical stage. LRP expression of lung cancer tissues (56.67％) was much higher than that of normal tissues (P＝0.000?4), and it was not associated with degree of cell differentiation, histological classification and the clinical stage. Of the 30 lung cancer specimens, 7 expressed all the three kinds of genes, and 10 expressed none of them. The coincident rate was 56.67％. Conclusion　The results suggest that MDR1, MRP and LRP gene may play important roles in drug resistance in NSCLC.