CAJ | 학술논문

利用PCR方法获得1?163?bp的戊型肝炎（Hepatitis E Virus, HEV）开放读码框架(Open Reading Frame, ORF) ORF2之3’大片段和369bp ORF3的完整片段，分别克隆到真核表达载体pcDNA3中，构建两种含有HEV 主要抗原表位的质粒DNA： pcE2和pcE3，分别或混合免疫Swiss小鼠三次（0时，第2周，第4周），观察其在小鼠体内诱发的体液免疫应答。ELISA检测结果表明，pcE2和pcE3在小鼠体内均可诱导出一定水平的HEV IgG抗体，且在第三次免疫接种两周后，100%的小鼠抗体阳转。与两种质粒单独免疫相比，两者同时注射的抗体水平较高。本研究为HEV DNA疫苗的研究打下一定基础。
이용PCR방법획득1?163?bp적무형간염（Hepatitis E Virus, HEV）개방독마광가(Open Reading Frame, ORF) ORF2지3’대편단화369bp ORF3적완정편단，분별극륭도진핵표체재체pcDNA3중，구건량충함유HEV 주요항원표위적질립DNA： pcE2화pcE3，분별혹혼합면역Swiss소서삼차（0시，제2주，제4주），관찰기재소서체내유발적체액면역응답。ELISA검측결과표명，pcE2화pcE3재소서체내균가유도출일정수평적HEV IgG항체，차재제삼차면역접충량주후，100%적소서항체양전。여량충질립단독면역상비，량자동시주사적항체수평교고。본연구위HEV DNA역묘적연구타하일정기출。
Two plasmid constructs, pcE2 and pcE3, containing 3' fragment of open reading frame 2 (ORF2,1163 bp) of hepatitis E virus (HEV) and full-length ORF3 (369 bp), were injected into bilateral tibialis of Swiss mice respectively，for three times (0, 2nd and 4th weeks) and observed the HEV IgG by ELISA. HEV IgG was induced after the injection of pcE2 or pcE3 or both, and the percentage of seraconversion was 100% after two weeks of the third injection. Compared with injection of either construct, the antibody titers were higher in the group with combined injection of two constructs.