解放军医学杂志
解放軍醫學雜誌
해방군의학잡지
MEDICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY
2001年
3期
163-165
,共3页
袁匀%王丽莉%张积仁
袁勻%王麗莉%張積仁
원균%왕려리%장적인
抗药性,多药%中性糖苷神经鞘脂类%基因,MDR
抗藥性,多藥%中性糖苷神經鞘脂類%基因,MDR
항약성,다약%중성당감신경초지류%기인,MDR
为研究糖脂合成酶抑制剂苯基棕榈酰胺吗啡丙醇（DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol*HCl，PPMP）对人恶性肿瘤多药耐药细胞株KBv200多药耐药mdr1基因的mRNA表达的调控，以及PPMP对细胞株KBv200的多药耐药性的逆转作用，作者应用体外细胞培养技术对细胞株KBv200进行PPMP处理，用RT-PCR技术分析PPMP处理前后细胞mdr1的mRNA表达，以流式细胞仪检测PPMP处理前后KBv200及其敏感株KB细胞内罗丹明-123的药物浓度变化。结果显示5μmol/L、15μmol/L PPMP可部分抑制KBv200细胞mdr1 mRNA表达，25μmol/L PPMP可完全抑制KBv200细胞mdr1 mRNA表达；PPMP可增加KBv200细胞内罗丹明荧光强度，随着PPMP浓度的增加，耐药细胞内的罗丹明荧光强度逐渐增大。提示PPMP对细胞株KBv200的多药耐药基因mdr1有抑制作用，并可以逆转KBv200的多药耐药性，逆转作用与PPMP浓度呈正相关。
為研究糖脂閤成酶抑製劑苯基棕櫚酰胺嗎啡丙醇（DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol*HCl，PPMP）對人噁性腫瘤多藥耐藥細胞株KBv200多藥耐藥mdr1基因的mRNA錶達的調控，以及PPMP對細胞株KBv200的多藥耐藥性的逆轉作用，作者應用體外細胞培養技術對細胞株KBv200進行PPMP處理，用RT-PCR技術分析PPMP處理前後細胞mdr1的mRNA錶達，以流式細胞儀檢測PPMP處理前後KBv200及其敏感株KB細胞內囉丹明-123的藥物濃度變化。結果顯示5μmol/L、15μmol/L PPMP可部分抑製KBv200細胞mdr1 mRNA錶達，25μmol/L PPMP可完全抑製KBv200細胞mdr1 mRNA錶達；PPMP可增加KBv200細胞內囉丹明熒光彊度，隨著PPMP濃度的增加，耐藥細胞內的囉丹明熒光彊度逐漸增大。提示PPMP對細胞株KBv200的多藥耐藥基因mdr1有抑製作用，併可以逆轉KBv200的多藥耐藥性，逆轉作用與PPMP濃度呈正相關。
위연구당지합성매억제제분기종려선알마배병순（DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol*HCl，PPMP）대인악성종류다약내약세포주KBv200다약내약mdr1기인적mRNA표체적조공，이급PPMP대세포주KBv200적다약내약성적역전작용，작자응용체외세포배양기술대세포주KBv200진행PPMP처리，용RT-PCR기술분석PPMP처리전후세포mdr1적mRNA표체，이류식세포의검측PPMP처리전후KBv200급기민감주KB세포내라단명-123적약물농도변화。결과현시5μmol/L、15μmol/L PPMP가부분억제KBv200세포mdr1 mRNA표체，25μmol/L PPMP가완전억제KBv200세포mdr1 mRNA표체；PPMP가증가KBv200세포내라단명형광강도，수착PPMP농도적증가，내약세포내적라단명형광강도축점증대。제시PPMP대세포주KBv200적다약내약기인mdr1유억제작용，병가이역전KBv200적다약내약성，역전작용여PPMP농도정정상관。
The current study was designed to investigate the effects of PPMP (DL-threo-1-phenyl-2- palmitoylamino-3-morpholino-1-propanol), a kind of glycolipids synthase inhibitor, on the modulation of mdr1 mRNA expression and the reversing effect of multi-drug resistance by PPMP in human malignancy KBv200cell line. In vitro KBv200 cells were treated with PPMP in different concentration, the alterations of mRNA expression of drug-resistant gene mdr1 in KB (sensitive cell line) and KBv200 (before and after the treatment of PPMP) cells were analyzed by RT-PCR. Intracellular rhodamine(Rh123) concentration was measured by flow cytometry. PPMP was found to inhibit mdr1 gene expression of KBv200 at the mRNA level, and complete inhibition appeared at 25μmol/L PPMP treatment for 48h. PPMP could increase intracellular Rh123 accumulation in resistant cell lines. This modulation of gene expression and Rh123 accumulation was directly correlated with the concentration of PPMP. It suggested that PPMP, a chemical inhibitor of glycolipids synthase, could modulate mdr1 expression at the mRNA level in a content dependent manner, PPMP possesses MDR-reversing activity.
