CAJ | 학술논문

目的：探索鼻咽癌细胞DNA合成的生物节律为临床制订鼻咽癌时辰化疗方案提供必要的参数。方法：BALB/C裸小鼠15只，置于程控的独立光照系统中（12h光照，12h黑暗）中同步化至少3周。然后双侧腋下接种人鼻咽低分化鳞癌细胞裸小鼠移植瘤CNE-2，成瘤后按灯亮后3、9、15、21h（即3、9、15、21HALO）的时间点取瘤块，制成单个细胞悬液,固定，染色后流式细胞仪测DNA含量。用SPSS软件系统ANOVA法检验各期细胞在4个时间点差异的显著性，用Cosinor法考察G1、S、G2/M期细胞在24h的分布是否符合余弦函数。结果：G1、S、G2/M期细胞在3、9、15、21HALO的分布随时间变化有显著性差异，G1、G2/M期细胞变化符合余弦节律。结论：移植于裸鼠的人鼻咽癌细胞的DNA合成随昼夜交替呈节律性变化。
목적：탐색비인암세포DNA합성적생물절률위림상제정비인암시신화료방안제공필요적삼수。방법：BALB/C라소서15지，치우정공적독립광조계통중（12h광조，12h흑암）중동보화지소3주。연후쌍측액하접충인비인저분화린암세포라소서이식류CNE-2，성류후안등량후3、9、15、21h（즉3、9、15、21HALO）적시간점취류괴，제성단개세포현액,고정，염색후류식세포의측DNA함량。용SPSS연건계통ANOVA법검험각기세포재4개시간점차이적현저성，용Cosinor법고찰G1、S、G2/M기세포재24h적분포시부부합여현함수。결과：G1、S、G2/M기세포재3、9、15、21HALO적분포수시간변화유현저성차이，G1、G2/M기세포변화부합여현절률。결론：이식우라서적인비인암세포적DNA합성수주야교체정절률성변화。
Objective： The circadian rhythm of DNA synthesis in nasopharyngeal carcinoma (NPC) cells was investigated so that to provide necessary data for making clinical chrono-chemotherapy schedule of NPC. Methods： Fifteen BALB/c mice were synchronized with an alternative lighting regimen with 12 hours for light and 12 hours for dark (12∶ 12 LD) at least 3 weeks. Human nasopharyngeal poorly differentiated squamous cell carcinoma(CNE-2) cells were implanted into both flanks of each mouse. Ten days after transplantation, sampling from tumor was conducted in the 3, 9, 15, 21 hours after light onset (HALO). Single cell suspension was obtained and stained with propidium iodide. The cellular DNA content was measured by flow cytometry. Data were documented by ANOVA and Cosinor analysis. Results： The proportion of tumor cells in Phage G1,S,G2-M varied according to circadian time with statistical significance. The distribution curves of Phage G1 and Phage G2-M fit for Cosinor changes. Conclusion： DNA synthesis of human nasopharyngeal poorly differentiated squamous cell carcinoma(CNE-2) cells trausplanted in nude mice varies according to circadian rhythm.