CAJ | 학술논문

目的毒性剂量H2O2可引起细胞坏死或凋亡, 此过程是否与H2O2引起的核［Ca2+］（［Ca2+］n）变化有关未见报导。本文研究牛磺酸对H2O2引起的血管平滑肌细胞（VSMC）［Ca2+］n与胞浆［Ca2+］(［Ca2+］c)的变化影响。方法应用激光共聚焦显微镜对负载Fluo-3的培养大鼠VSMC的［Ca2+］n及［Ca2+］c变化进行研究。结果在0.5% H2O2作用下，［Ca2+］n与［Ca2+］c持续升高，用抗氧化细胞保护剂-牛磺酸20 mmol*L-1预处理细胞，可降低H2O2引起的［Ca2+］n 升幅 (P<0.05)，但不影响［Ca2+］c升幅(P>0.05)，表明［Ca2+］n变化与［Ca2+］c变化对牛磺酸反应性存在差异。结论 VSMC的核Ca2+调控与胞浆Ca2+调控各自具有一定的独立性。牛磺酸对H2O2引起的大鼠VSMC核Ca2+的变化具有保护作用。
목적독성제량H2O2가인기세포배사혹조망, 차과정시부여H2O2인기적핵［Ca2+］（［Ca2+］n）변화유관미견보도。본문연구우광산대H2O2인기적혈관평활기세포（VSMC）［Ca2+］n여포장［Ca2+］(［Ca2+］c)적변화영향。방법응용격광공취초현미경대부재Fluo-3적배양대서VSMC적［Ca2+］n급［Ca2+］c변화진행연구。결과재0.5% H2O2작용하，［Ca2+］n여［Ca2+］c지속승고，용항양화세포보호제-우광산20 mmol*L-1예처리세포，가강저H2O2인기적［Ca2+］n 승폭 (P<0.05)，단불영향［Ca2+］c승폭(P>0.05)，표명［Ca2+］n변화여［Ca2+］c변화대우광산반응성존재차이。결론 VSMC적핵Ca2+조공여포장Ca2+조공각자구유일정적독립성。우광산대H2O2인기적대서VSMC핵Ca2+적변화구유보호작용。
AIM　As one of the reactive oxygen species, toxic dose of H2O2 leads to the necrosis or apoptosis of many kinds of cells. It is nuclear whether these processes are related with the changes of nuclear Ca2+ elicited by H2O2. This study on the effect of taurine on changes of nuclear and cytosolic (Ca2+) elicited by H2O2 in rat vascular smooth muscle cells. METHODS　The techniques of Fluo-3 and laser scanning confocal microscopy were used. RESULTS　Rapid and sustaining rise of nuclear ［Ca2+］ (［Ca2+］n) and cytosolic ［Ca2+］(［Ca2+］c) were found in cultured rat vascular smooth muscle cells upon adding of 0.5% H2O2. Pretreatment of the cells with 20 mmol*L-1 taurine, a regulator of cellular Ca2+ homestasis, significantly decreased the rise amplitude of ［Ca2+］n (P<0.05), but failed to affect that of ［Ca2+］c (P>0.05) elicited by H2O2. It was suggested that the changes of ［Ca2+］n and ［Ca2+］c have different respond to taurine. CONCLUSION　It was concluded that there exist the independent regulating mechanisms of Ca2+ in the nucleus from differences between the nucleus and cytosol Ca2+ in sensitivity to action of H2O2 and taurine.