CAJ | 학술논문

通过物料衡算和线性规划方法建立了进行代谢流分析的数学模型，并首次应用于批式培养过程.分析结果表明，细胞对葡萄糖等底物的代谢效率很低，细胞对谷氨酰胺等氨基酸浓度的变化响应迟缓，在底物充裕的迟滞期及谷氨酰胺限制条件下的代谢效率高于对数生长期.另外限制谷氨酰胺等氨基酸进入能量代谢，既可减少氨的积累，也会减少乳酸的生成.
통과물료형산화선성규화방법건립료진행대사류분석적수학모형，병수차응용우비식배양과정.분석결과표명，세포대포도당등저물적대사효솔흔저，세포대곡안선알등안기산농도적변화향응지완，재저물충유적지체기급곡안선알한제조건하적대사효솔고우대수생장기.령외한제곡안선알등안기산진입능량대사，기가감소안적적루，야회감소유산적생성.
An improved mathematical model of metabolic flux analysis bymaterial balances and linear programming method was established, which was first applied to batch culture process in this paper. The analysis results indicate that the metabolic efficiency of substrates such as glucose is very low. It showed that the response of cell metabolism to the step change of glutamine and other amino acid concentration was time-delayed. Metabolic efficiency was higher both in lag phase with highest substrate concentrations and glutamine-limited phase than that in exponential phase. It was also concluded that restraining glutamine from entering energy metabolism could reduce the accumulation of both ammonia and lactate.