中国生物制品学杂志
中國生物製品學雜誌
중국생물제품학잡지
CHINESE JOURNAL OF BIOLOGICALS
2000年
4期
241-244
,共4页
熊仁平%周元国%刘苹%侯淦泉
熊仁平%週元國%劉蘋%侯淦泉
웅인평%주원국%류평%후감천
胸腺素 哮喘 G蛋白 Western blot
胸腺素 哮喘 G蛋白 Western blot
흉선소 효천 G단백 Western blot
Thymosin Asthma G protein Western blot
目的 提取胸腺素并检测药物指标，观察其临床应用和动物实验效果，探讨胸腺素对哮喘豚鼠肺组织Gα表达的影响。方法 采用酸碱沉淀加离心法制备胸腺素，经多肽定量、活性鉴定、细菌培养、毒性试验和热原实验，临床疗效观察，并以卵蛋白雾化吸入致敏法复制哮喘模型，Western blot分析法测定哮喘豚鼠肺组织Gα水平。结果 所制胸腺素符合中国药典标准。临床疗效确切，经治疗后，哮喘豚鼠肺组织Giα、Gqα水平显著降低(P＜0.05)，Gsα无显著变化。结论 采用酸碱沉淀加离心法可制备胸腺素，其治疗哮喘动物的机理可能是通过改变肺组织Gα水平来达到。
目的 提取胸腺素併檢測藥物指標，觀察其臨床應用和動物實驗效果，探討胸腺素對哮喘豚鼠肺組織Gα錶達的影響。方法 採用痠堿沉澱加離心法製備胸腺素，經多肽定量、活性鑒定、細菌培養、毒性試驗和熱原實驗，臨床療效觀察，併以卵蛋白霧化吸入緻敏法複製哮喘模型，Western blot分析法測定哮喘豚鼠肺組織Gα水平。結果 所製胸腺素符閤中國藥典標準。臨床療效確切，經治療後，哮喘豚鼠肺組織Giα、Gqα水平顯著降低(P＜0.05)，Gsα無顯著變化。結論 採用痠堿沉澱加離心法可製備胸腺素，其治療哮喘動物的機理可能是通過改變肺組織Gα水平來達到。
목적 제취흉선소병검측약물지표，관찰기림상응용화동물실험효과，탐토흉선소대효천돈서폐조직Gα표체적영향。방법 채용산감침정가리심법제비흉선소，경다태정량、활성감정、세균배양、독성시험화열원실험，림상료효관찰，병이란단백무화흡입치민법복제효천모형，Western blot분석법측정효천돈서폐조직Gα수평。결과 소제흉선소부합중국약전표준。림상료효학절，경치료후，효천돈서폐조직Giα、Gqα수평현저강저(P＜0.05)，Gsα무현저변화。결론 채용산감침정가리심법가제비흉선소，기치료효천동물적궤리가능시통과개변폐조직Gα수평래체도。
Objective To extract thymosin, detect the pharmaceutical indexes of it,observe the effect ofit in clinical application and animal test, and explore the influence of it on the expression of Ga protein in thelung tissue of guinea pig with asthma. Methods Thymosin was prepared by acid-base precipitation and cen-trifugation, and the pharmaceutical indexes of it were detected by quantification of polypeptide, identification ofactivity, bacterial culture, toxicity test and pyrogen test. The clinical effect of it was observed. Guinea-pig modelwith asthma was established by aerosol inhalation of egg albumin. The Ga protein level in the lung tissue ofguinea pig with asthma was analyzed by Western blot. Results The thymosin met the requirement of ChinesePharmacopeia. The clinical effect of it was definite. The Gia and Gqa levels in the lung tissues of guinea-pigswere significantly decreased after being treated with it (p＜0.5). However, no significant change of Gsa levelwas observed. Conclusiou The thymosin prepared by acid-base precipitation and centrifugation can be appliedin clinic. The curative mechanism of it might be changing the Ga protein level in lung tissue.
