应用与环境生物学报
應用與環境生物學報
응용여배경생물학보
CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
2000年
5期
423-427
,共5页
钟蓉%董微%肖邡明%袁水泉%吴伯骥
鐘蓉%董微%肖邡明%袁水泉%吳伯驥
종용%동미%초방명%원수천%오백기
籼粳杂种%不育突变体%育性%RAPD%RFLP
秈粳雜種%不育突變體%育性%RAPD%RFLP
선갱잡충%불육돌변체%육성%RAPD%RFLP
Indica-japonica rice hybrid%sterile mutant%fertility%R APD%RFLP
91FS是一个稳定遗传的籼粳杂种水稻新型不育突变体,育性特征表现为,雌雄配子和受精过程正常,约25%的受精卵发育成胚,约75%的受精卵终止发育,与91FS 群体约25%的结实率吻合,而且六代保持不变;91FS的RAPD和RFLP的谱带六代保持稳定和一致,与91FS的杂种性状和育性表达的稳定性也是吻合的;91FS与籼粳杂种可育材料91FM和以 91 FS为父本的杂交F1代(D297A × 91FS)材料的RAPD比较分析表明,91FS与CK相比表现出广泛的DNA多态性和同一性,同时也表现出特异的新带和一些缺失谱带,其多态性、同一性和特异性是可遗传的. 图4 表1 参15
91FS是一箇穩定遺傳的秈粳雜種水稻新型不育突變體,育性特徵錶現為,雌雄配子和受精過程正常,約25%的受精卵髮育成胚,約75%的受精卵終止髮育,與91FS 群體約25%的結實率吻閤,而且六代保持不變;91FS的RAPD和RFLP的譜帶六代保持穩定和一緻,與91FS的雜種性狀和育性錶達的穩定性也是吻閤的;91FS與秈粳雜種可育材料91FM和以 91 FS為父本的雜交F1代(D297A × 91FS)材料的RAPD比較分析錶明,91FS與CK相比錶現齣廣汎的DNA多態性和同一性,同時也錶現齣特異的新帶和一些缺失譜帶,其多態性、同一性和特異性是可遺傳的. 圖4 錶1 參15
91FS시일개은정유전적선갱잡충수도신형불육돌변체,육성특정표현위,자웅배자화수정과정정상,약25%적수정란발육성배,약75%적수정란종지발육,여91FS 군체약25%적결실솔문합,이차륙대보지불변;91FS적RAPD화RFLP적보대륙대보지은정화일치,여91FS적잡충성상화육성표체적은정성야시문합적;91FS여선갱잡충가육재료91FM화이 91 FS위부본적잡교F1대(D297A × 91FS)재료적RAPD비교분석표명,91FS여CK상비표현출엄범적DNA다태성화동일성,동시야표현출특이적신대화일사결실보대,기다태성、동일성화특이성시가유전적. 도4 표1 삼15
The fertility characteristic of 91FS, a new heritable Indica-japonica rice hybrid sterile mutant, displayed its normal female/male gametes and f ertilization process. About 25% of the fertilized eggs could develop into mature embryos while about 75% of the fertilized eggs stopped further developing, whic h coincided with the population seed percentage of 91FS. In addition, this featu re kept constant within six generations. The stability and consistency of 91FS R FLP bands coincided with the stability of hybrid characteristic and fertility ex pression. The RAPD results of 91FS, 91FM(a fertile Indica-japonica rice hyb rid) and F1(D297AX91FS) showed that there were quite different kinds of DNA po lymorphism among 91FS and the other two controls. 91FS also displayed some new s pecial DNA bands and absent bands. The polymorphism, identity and specificity of 91FS are heritable. Fig 4, Tab 1, Ref 15