CAJ | 학술논문

以甲壳素的浓盐酸水解产物氨基葡萄糖盐酸盐为原料,通过阴离子交换层析法制备氨基葡萄糖硫酸盐,并对其的部分性质进行研究.采用本方法制备的氨基葡萄糖硫酸盐产物的氨基葡萄糖含量为71.60%,硫酸根含量为18.25%,氯离子含量为0.038%.经纸层析鉴定,以吡啶、乙酸乙酯、水和冰醋酸按体积比5:5:3:l组成的溶剂系统进行展层,氨基葡萄糖硫酸盐的Rf值为0.36.将氨基葡萄糖硫酸盐产物的晶体重新溶解于20℃水中时,它的比旋光度随时间的延长而下降,1.5h后趋于稳定,从92.1°降低至56.7°.对它的红外光谱鉴定表明,在波数为3303.9、1415.6、1215.6、1181.2、1118.7、1093.7和1034.4 cm-1处有特征吸收峰.通过对氨基葡萄糖硫酸盐产物的元素分析,碳、氢、氮和硫的含量分别为29.63%、6.34%、5.39%和6.56%.
이갑각소적농염산수해산물안기포도당염산염위원료,통과음리자교환층석법제비안기포도당류산염,병대기적부분성질진행연구.채용본방법제비적안기포도당류산염산물적안기포도당함량위71.60%,류산근함량위18.25%,록리자함량위0.038%.경지층석감정,이필정、을산을지、수화빙작산안체적비5:5:3:l조성적용제계통진행전층,안기포도당류산염적Rf치위0.36.장안기포도당류산염산물적정체중신용해우20℃수중시,타적비선광도수시간적연장이하강,1.5h후추우은정,종92.1°강저지56.7°.대타적홍외광보감정표명,재파수위3303.9、1415.6、1215.6、1181.2、1118.7、1093.7화1034.4 cm-1처유특정흡수봉.통과대안기포도당류산염산물적원소분석,탄、경、담화류적함량분별위29.63%、6.34%、5.39%화6.56%.
By using anion-exchange column chromatography, glucosamine sulfate was prepared from glucosamine hydrochloride that was produced by acidic hydrolysis of chitin and some properties of the product were studied. The contents of glucosamine, sulfate radical and chloridion in the product were 71. 60%, 18.25% and 0.038%, respectively. The Rf value of the glucosamine sulfate was 0.36 measured by paper chromatography with the solvent system of pyridine, ethyl acetate, water and glacial acetic acid (5:5:3:1 in volume). While redissolved in water at 20℃, the specific rotation of glucosamine sulfate decreased with the time from 92.1° to about 56.7° and was, however, stable after 1.5 h at 56.7°. It exhibited the characteristic absorption bands at 3307.9, 1415.6, 1215.6, 1181.2, 1118.7, 1093.7 and 1034.4 cm-1 in IR spectrum. The elemental analysis for the glucosamine suffate showed that the contents of carbon, hydrogen, nitrogen and sulfur were 29.63, 6.34, 5.39 and 6.56 %, respectively.