宁波高等专科学校学报
寧波高等專科學校學報
저파고등전과학교학보
JOURNAL OF NINGBO COLLEGE
2001年
z1期
147-150
,共4页
施青红%吴东亮%陈志斌%叶明立%李明%凌艳艳%朱岩
施青紅%吳東亮%陳誌斌%葉明立%李明%凌豔豔%硃巖
시청홍%오동량%진지빈%협명립%리명%릉염염%주암
吲哚-3-乙酸%吲哚-3-丁酸%离子色谱法%荧光检测器
吲哚-3-乙痠%吲哚-3-丁痠%離子色譜法%熒光檢測器
신타-3-을산%신타-3-정산%리자색보법%형광검측기
吲哚-3-乙酸和吲哚-3-丁酸在离子色谱仪Dionex 4000i上使用荧光检测器进行检测。该测定采用1.0ml/min的0.45V／V CH30H，0.01mol/1Na2CO3的混合溶液作为淋洗液在dinoer OmniPacPAX500柱分离。吲哚-3-乙酸和吲哚-3-丁酸的检测限分别为0.045μg/ml和0.167μg/ml，有良好的重现性和线性关系。对土壤样品进行测定，有较好的回收率。
吲哚-3-乙痠和吲哚-3-丁痠在離子色譜儀Dionex 4000i上使用熒光檢測器進行檢測。該測定採用1.0ml/min的0.45V／V CH30H，0.01mol/1Na2CO3的混閤溶液作為淋洗液在dinoer OmniPacPAX500柱分離。吲哚-3-乙痠和吲哚-3-丁痠的檢測限分彆為0.045μg/ml和0.167μg/ml，有良好的重現性和線性關繫。對土壤樣品進行測定，有較好的迴收率。
신타-3-을산화신타-3-정산재리자색보의Dionex 4000i상사용형광검측기진행검측。해측정채용1.0ml/min적0.45V／V CH30H，0.01mol/1Na2CO3적혼합용액작위림세액재dinoer OmniPacPAX500주분리。신타-3-을산화신타-3-정산적검측한분별위0.045μg/ml화0.167μg/ml，유량호적중현성화선성관계。대토양양품진행측정，유교호적회수솔。
indoleacetic acid and indolebutyric acid in soil are detected by Dionex 4000i ion chrromatography with fluorescence detector. This experiment is performed by OmniPac PAX - 500 column using 0. 010mol/L Na2CO3, 0.45V/V CH30H as eluate. The fluorescene detector was measured in excitation and emission wavelengths of 285nm , 360nm, respectively. Tne detecting limit is 0. 045μg/mL 和 O. 167μ g/mL for indoleacetic acid and indolebutyrie acid, respectively, The method with linering、 selection and sensitivity can be used in trace amount indoleacetic acid and indolebutyric acid in soil samples.
