湖北农业科学
湖北農業科學
호북농업과학
2009年
11期
2625-2626,2695
,共3页
CBM%非变性亲和电泳%结合特性
CBM%非變性親和電泳%結閤特性
CBM%비변성친화전영%결합특성
CBM%non-denaturing affinity electrophoresis (NDAE)%Binding specificity
以多种碳水化合物为底物,通过非变性亲和电泳(NDAE)来检验Tt APuX21是否能与碳水化合物结合,以证明Tt APuX21是否确为有功能的碳水化合物结合结构域.NDAE的分析结果表明,Tt A-PuX21能与水溶性淀粉、糊精和普鲁兰糖结合,Tt APuX21是一个有功能的碳水化合物结合结构域.
以多種碳水化閤物為底物,通過非變性親和電泳(NDAE)來檢驗Tt APuX21是否能與碳水化閤物結閤,以證明Tt APuX21是否確為有功能的碳水化閤物結閤結構域.NDAE的分析結果錶明,Tt A-PuX21能與水溶性澱粉、糊精和普魯蘭糖結閤,Tt APuX21是一箇有功能的碳水化閤物結閤結構域.
이다충탄수화합물위저물,통과비변성친화전영(NDAE)래검험Tt APuX21시부능여탄수화합물결합,이증명Tt APuX21시부학위유공능적탄수화합물결합결구역.NDAE적분석결과표명,Tt A-PuX21능여수용성정분、호정화보로란당결합,Tt APuX21시일개유공능적탄수화합물결합결구역.
This study aimed to investigate whether Tt APuX21 was a functional carbohydrate-binding module (CBM). In order to test whether Tt APuX21 possessed affinity for polysaccharides, non-denaturing affinity electrophoresis (NDAE) was carried out using different carbohydrates as ligands. NDAE displayed that Tt APuX21 had affinity for soluble starch, dextrin and pullulan. The results showed that Tt APuX21 was a functional carbohydrate-binding module.
