CAJ | 학술논문

建立了表面活性剂增敏荧光光度法测定牛奶中三聚氰胺的方法.利用弱碱性介质中阳离子表面活性剂(CTMAB)对三聚氰胺荧光强度的增敏作用,在pH=8.0的Tris-HCl缓冲溶液中,以CTMAB为增敏剂,测定三聚氰胺,线性范围为25～1000 μg/L,检出限为19 μg/L,相对标准偏差为1.6%.按国标方法处理样品,采用本法测定,回收率偏高;利用自制固相萃取整体柱对牛奶样品进行预处理后,实际样品检测获得满意结果.此方法简便、快捷、准确,可用于大量牛奶样品中三聚氰胺的快速初筛及检测.
건립료표면활성제증민형광광도법측정우내중삼취청알적방법.이용약감성개질중양리자표면활성제(CTMAB)대삼취청알형광강도적증민작용,재pH=8.0적Tris-HCl완충용액중,이CTMAB위증민제,측정삼취청알,선성범위위25～1000 μg/L,검출한위19 μg/L,상대표준편차위1.6%.안국표방법처리양품,채용본법측정,회수솔편고;이용자제고상췌취정체주대우내양품진행예처리후,실제양품검측획득만의결과.차방법간편、쾌첩、준학,가용우대량우내양품중삼취청알적쾌속초사급검측.
Melamine is a kind of triazine compound and the fluorescence of it can get enhanced in the presence of cationic surfactant in weak alkaline medium. A new fluorescent spectrophotometry based on this principle) has been developed to determine melamine under the optimum conditions such as Tris-HCl buffer solution), pH 8.0 and with CTMAB as sensitizing agent. The linear range, detection limit and relative standard deviation were 25-1000 μg/L, 19 μg/L and 1.6%,respectively. The samples were pretreated according to the solid phase extraction monolithic column to carry out the detection of real milk. This method is simple, rapid and accurate. It can be used to screen and detect the milk samples primarily.