CAJ | 학술논문

番茄黄化曲叶病毒病是一种由烟粉虱传播的病毒病,给番茄生产造成严重威胁.2009年在北京郊区调查时发现部分保护地种植的番茄植株表现典型黄化曲叶症状.通过提取典型症状样品总DNA利用粉虱传双生病毒检测简并引物PA/PB,进行PCR扩增到541 bp的特异条带.通过测序和核苷酸序列比对表明该序列与番茄黄化曲叶病毒序列相似性最高为99%.分子检测结果表明北京郊区部分保护地种植的番茄已被烟粉虱传播的番茄黄化曲叶病毒侵染危害.
번가황화곡협병독병시일충유연분슬전파적병독병,급번가생산조성엄중위협.2009년재북경교구조사시발현부분보호지충식적번가식주표현전형황화곡협증상.통과제취전형증상양품총DNA이용분슬전쌍생병독검측간병인물PA/PB,진행PCR확증도541 bp적특이조대.통과측서화핵감산서렬비대표명해서렬여번가황화곡협병독서렬상사성최고위99%.분자검측결과표명북경교구부분보호지충식적번가이피연분슬전파적번가황화곡협병독침염위해.
Tomato yellow leaf curl disease is one of the devastating diseases of cultivated tomato (Lycopersicon esculentum Mill.) and caused by Tomato yellow leaf curl virus (TYLCV) which is transmitted by Bemisia tabaci (Gennadius). In 2009, some tomato plants showing typical yellow leaf curl symptom were found in greenhouses in rural areas of Beijing. With total DNA isolated from diseased samples and the primers PA/PB specific to gemini-viruses genome, a 541bp fragment was obtained by PCR. The nucleotide sequence of the fragment has the highest identity (99%) with that of Tomato yellow leaf curl virus isolate JSNJ1. This finding indicated that some tomato plants in Beijing have been infected by TYLCV.