CAJ | 학술논문

通过42个单位点SSR标记分析59个甘蓝型油菜品种,比较春性和半冬性甘蓝型油菜品种间的遗传差异.结果表明,不同位点上存在2～6个等位基因,平均每位点检测到4.29个等位基因.不同位点的遗传多样性指数在0.40～0.70之间,所有供试位点平均遗传多样性指数为0.50,表明甘蓝型油菜品种资源中存在丰富的遗传变异.春性和半冬性油菜品种间的等位基因总数相差不大,主要等位基因的类型和频率存在明显的差异,而且春性和半冬性品种中分别存在相当数量的特异稀有等位基因.春性和半冬性油菜品种在27个位点上的遗传分化水平很小,仅在2个位点上存在明显的遗传分化,这些分化主要是由品种的春化特性差异造成.聚类分析表明春性和半冬性品种之间存在广泛的亲缘关系.
통과42개단위점SSR표기분석59개감람형유채품충,비교춘성화반동성감람형유채품충간적유전차이.결과표명,불동위점상존재2～6개등위기인,평균매위점검측도4.29개등위기인.불동위점적유전다양성지수재0.40～0.70지간,소유공시위점평균유전다양성지수위0.50,표명감람형유채품충자원중존재봉부적유전변이.춘성화반동성유채품충간적등위기인총수상차불대,주요등위기인적류형화빈솔존재명현적차이,이차춘성화반동성품충중분별존재상당수량적특이희유등위기인.춘성화반동성유채품충재27개위점상적유전분화수평흔소,부재2개위점상존재명현적유전분화,저사분화주요시유품충적춘화특성차이조성.취류분석표명춘성화반동성품충지간존재엄범적친연관계.
42 single-locus SSR markers were used to analyze the genetic diversity of 59 spring and weak-winter rapeseed (Brassica napus L.) cultivars. The results indicated that the allele number varied from 2 to 6 with an average of 4.29 alleles each. The diversity index varied from 0.40 to 0.70 with an average of 0.50. It indicated abundant genetic variation in the germplasm. The difference of total number of alleles was not statistically significant between spring and weak-winter cultivars. Distinct types of major alleles were observed in the spring and weak-winter cultivars, and their corresponding allele frequencies were significantly different between the spring and weak-winter cultivars. Uncommon alleles were identified. Little genetic differentiations were detected between the spring and weak-winter cultivars at 27 loci. 2 loci had significant differentiations, which were caused by vernalization. Cluster analysis could not completely separate the spring and weak-winter cultivars, which suggested that they have broad genetic relationship. The present results would provide valuable guidance for germplasm collection and genetic breeding in B.napus.