CAJ | 학술논문

目的研究smad2/3在沙鼠脑缺血再灌注脑内表达及氟桂利嗪干预后对其表达的影响.方法 35只沙鼠随机分假手术组、缺血再灌注组和氟桂利嗪治疗组3组,采用夹闭双侧颈总动脉制作沙鼠脑缺血再灌注模型,于缺血10 min再灌注1 d、3 d、7 d各时间点处死动物,用免疫组化方法检测各组沙鼠脑内smad2/3的表达情况.结果假手术组沙鼠脑内smad2/3弱阳性表达,而缺血再灌注1 d,3 d,7 d后沙鼠脑内呈阳性或强阳性表达,两者比较差异有统计学意义(P<0.01);氟桂利嗪治疗组smad2/3的表达低于缺血再灌注组,2组比较差异有统计学意义(P<0.05).结论沙鼠脑内有smad2/3表达,脑缺血再灌注后,脑内smad2/3表达上调;氟桂利嗪干预后,其表达下调.
목적 연구smad2/3재사서뇌결혈재관주뇌내표체급불계리진간예후대기표체적영향.방법 35지사서수궤분가수술조、결혈재관주조화불계리진치료조3조,채용협폐쌍측경총동맥제작사서뇌결혈재관주모형,우결혈10 min재관주1 d、3 d、7 d각시간점처사동물,용면역조화방법검측각조사서뇌내smad2/3적표체정황.결과 가수술조사서뇌내smad2/3약양성표체,이결혈재관주1 d,3 d,7 d후사서뇌내정양성혹강양성표체,량자비교차이유통계학의의(P<0.01);불계리진치료조smad2/3적표체저우결혈재관주조,2조비교차이유통계학의의(P<0.05).결론 사서뇌내유smad2/3표체,뇌결혈재관주후,뇌내smad2/3표체상조;불계리진간예후,기표체하조.
Objective To study the expression of smad2/3 protein and the effects of flunarizine on the its expression in braln tissue following transient cerebral ischemie reperfusion in gerbils. Methods A cerebral ischemia-reperfusion model in gerbils was established by clamping both common carotids. Thirty-five gerbils were randomly divided into three groups, sham operation group, cerebral ischemia-reperfusion group and flunarizine treatment group. The expression of smad2/3 protein in brain tissue was detected by immunohistochemistry technique. Results Experimental results revealed that smad2/3 protein was expressed in neuroeyte in 35 gerbil brain. Compared with sham operation group, the expression of smad2/3 protein in neurecytes of cerebral isehemia-reperfusion group was evidently increased at the lst day, 3rd day and 7th day (P <0. 01). Compared with cerebral ischemia-reperfusion group, the expression of smad2/3 protein in neurecytes of gerbils in flunarizine treatment group was evidently decreased at these time point (P < 0.05). Condusions Smad2/3 protein was expressed in nettrcvytes of gerbils. Expression of smad2/3 protein in neuroeytes of gerbils was evidently increased following cerebral ischemic reperfusion, and its expression in flunarizine treatment group was evidently decreased.