CAJ | 학술논문

目的研究单克隆永生化骨髓基质干细胞在体内和体外多向分化的差异性.方法对挑取的单克隆细胞系和亲代细胞系分别在体外进行脂肪、骨、神经样细胞诱导分化;接种联合免疫缺陷性鼠,对移植物进行免疫组化染色;比较体内分化的不同.结果在体外诱导分化中,单克隆细胞系丢失成骨分化的能力;体内分化中,人永生化骨髓基质细胞(HMSC-TERT)-2较其他细胞系在人白细胞共同抗原,神经胶质酸性蛋白,波形蛋白表达上明显增强;HMSC-TERT-C19较HMSC-TERT-2,HMSC-TERT-20以及MSC-H在角蛋白表达上明显增强;HMSC-TERT-C2比HMSC-TERT-2在肌动蛋白表达上明显增强.结论人永生化骨髓基质细胞单克隆细胞系在体内和体外的分化存在差异性.提示标准化体外培养的条件和体内接种的程序,对早日将干细胞应用于临床有重要的意义.
목적 연구단극륭영생화골수기질간세포재체내화체외다향분화적차이성.방법 대도취적단극륭세포계화친대세포계분별재체외진행지방、골、신경양세포유도분화;접충연합면역결함성서,대이식물진행면역조화염색;비교체내분화적불동.결과 재체외유도분화중,단극륭세포계주실성골분화적능력;체내분화중,인영생화골수기질세포(HMSC-TERT)-2교기타세포계재인백세포공동항원,신경효질산성단백,파형단백표체상명현증강;HMSC-TERT-C19교HMSC-TERT-2,HMSC-TERT-20이급MSC-H재각단백표체상명현증강;HMSC-TERT-C2비HMSC-TERT-2재기동단백표체상명현증강.결론 인영생화골수기질세포단극륭세포계재체내화체외적분화존재차이성.제시표준화체외배양적조건화체내접충적정서,대조일장간세포응용우림상유중요적의의.
Objective To study the clonal heterogeneity in differentiation potential of immortalized mesenchymal stem cells in vitro and in rive.Methods The monoclonal cell lines were performed with limiting dilution cloning,and were induced to adipocytic,asteogenic and neuronal differentiation in vitro.After transplanted the monoclonal cell lines into SCID mice,the xenotransplants were removed and evaluated by immunohistochemistry.Results From the parental HMSC-TERT,32 single-cell derived clones were established,of which the differentiation properties varied considerably in vitro.The cells grow in different plating densities during expansion in culture:HMSC-TERT-2 expressed more strongly in LCA,GFAP and vimemin;HMSC-TERT-C19 expressed more strongly in keratinose than HMSC-TERT-2,HMSC-IERT-20,and MSC-H;HMSC-TERT-C2 expressed more strongly in actin than HMSC-TERT-2.Conclusions The HMSC-TERT monoclone cells are heterogeneity in differentiation petential in vitro and in vivo,suggesting that standard in vitro culture and in vivo inoculate procedure phy an important role in the clinical application of stem cells.