CAJ | 학술논문

目的观察联合激动Toll样受体(TLRs)的树突细胞对小鼠结肠癌的杀伤作用以及荷瘤小鼠的生存时间.方法用转染人癌胚抗原(CEA)的MC38小鼠结肠癌细胞株(MC38-CEA)和MC38细胞株(1×106个/只)皮下注射人CEA转基因小鼠,7d后将不同的树突状细胞(DC)疫苗(1×106个/只)于对侧皮下注射小鼠,每周2次测量各组小鼠肿瘤的垂直直径,并记录小鼠的生存时间,绘制各组肿瘤生长曲线和小鼠生存曲线.结果在预防性疫苗干预的MC38-CEA肿瘤模型中,第41天联合TLR且荷载CEA肽段的DC(TLR-DC+ CEA)组小鼠肿瘤平均体积(1091.20±226.12) mm3明显小于其余各组(P＜0.01),并且存活期明显优于其余各组(P＜0.01)；在治疗性疫苗干预时,第41天TLR-DC+ CEA组小鼠肿瘤平均体积(6487.20±1024.47) mm3,小于磷酸盐缓冲液(PBS)对照组(9361.20±2654.64) mm3(P＜0.05),而与其余对照组差异无统计学意义(P＞0.05)；在小鼠MC38肿瘤模型中,各组肿瘤平均体积差异无统计学意义(P＞0.05),且各组小鼠存活期差异无统计学意义(P＞0.05).结论联合激动TLR的DC疫苗能抑制表达CEA的小鼠结肠癌生长并延长小鼠生存期.
목적 관찰연합격동Toll양수체(TLRs)적수돌세포대소서결장암적살상작용이급하류소서적생존시간.방법 용전염인암배항원(CEA)적MC38소서결장암세포주(MC38-CEA)화MC38세포주(1×106개/지)피하주사인CEA전기인소서,7d후장불동적수돌상세포(DC)역묘(1×106개/지)우대측피하주사소서,매주2차측량각조소서종류적수직직경,병기록소서적생존시간,회제각조종류생장곡선화소서생존곡선.결과 재예방성역묘간예적MC38-CEA종류모형중,제41천연합TLR차하재CEA태단적DC(TLR-DC+ CEA)조소서종류평균체적(1091.20±226.12) mm3명현소우기여각조(P＜0.01),병차존활기명현우우기여각조(P＜0.01)；재치료성역묘간예시,제41천TLR-DC+ CEA조소서종류평균체적(6487.20±1024.47) mm3,소우린산염완충액(PBS)대조조(9361.20±2654.64) mm3(P＜0.05),이여기여대조조차이무통계학의의(P＞0.05)；재소서MC38종류모형중,각조종류평균체적차이무통계학의의(P＞0.05),차각조소서존활기차이무통계학의의(P＞0.05).결론 연합격동TLR적DC역묘능억제표체CEA적소서결장암생장병연장소서생존기.
Objective To observe the anti-tumor effect of dentritic cells (DC) vaccine activated by combined Toll-like receptor (TLR) on murine colon cancer model and the survival time of tumor-bearing mice.Methods Murine colon cancers for the experiment were established using MC38-CEA and MC38 cells (1 × 106 cells per mouse).Seven days later,different DC vaccines (1 × 106 cells per mouse) were injected subcutaneously.The growth status of the subcutaneous tumors was observed twice per week and the survival was recorded.Finally the mean tumor volume in each group was analyzed for plotted curves and survival curves of mice in each group were depicted.Results In the MC38-CEA murine tumor model with preventive DC vaccine,mean tumor volume in the group of TLR activated DC pulsed with CEA peptide [ (1091.20 ±226.12) mm3] was significantly smaller than that of other groups (P ＜ 0.01 ) and the survival was significantly longer (P ＜ 0.01 ) ; When with therapeutic DC vaccine,the mean tumor volume of TLR + CEA-DC group [ (6487.20 ± 776.18 ) mm3 ] on the day 41 was smaller than that of PBS group [ (9361.20 ±2654.64) mm3 ] (P ＜0.05),but there was no difference from other groups (P ＞0.05).In the murine tumor model with MC38 colon cancer cells,mean tumor volume in all groups had no significant difference (P ＞ 0.05 ),and the survival time in each group had no significant difference ( P ＞ 0.05 ).Conclusion Combined TLR activated DC vaccine can inhibit the growth of MC38-CEA colon cancer cells and prolong survival days.