CAJ | 학술논문

目的在兔腰椎椎体钻孔种植人型H37 Rv结核标准菌株构建兔脊柱结核模型.方法对16只兔腰5椎体上方钻孔,填充明胶海绵,吸附结核菌混悬液0.1 ml(菌量:5g/L)骨蜡封闭钻孔,在术后不同时间段行大体观察、影像学、组织病理学、细菌学等检查.结果 16只大白兔中,其中13只兔腰椎椎体感染结核菌后,局部反应较明显,全身反应较轻,完成实验；3只未感染结核杆菌.在感染的大白兔中,2只出现消瘦、厌食等症状；术后1个月X线阳性4只,术后2个月X线阳性8只、CT阳性12只、MRI阳性13只；感染大白兔的椎体、椎旁软组织切片苏木素-伊红(HE)染色示有脓细胞、类上皮样细胞或坏死灶等形成,对形成的脓肿进行脓液培养显示69.2％结核分枝杆菌生长.建立模型成功率为69.2％.结论通过在兔脊柱椎体局部进行适当剂量H37 Rv结核标准菌株的种植感染,可构建出与人类脊柱结核病理变化相似的兔脊柱结核.
목적 재토요추추체찬공충식인형H37 Rv결핵표준균주구건토척주결핵모형.방법 대16지토요5추체상방찬공,전충명효해면,흡부결핵균혼현액0.1 ml(균량:5g/L)골사봉폐찬공,재술후불동시간단행대체관찰、영상학、조직병이학、세균학등검사.결과 16지대백토중,기중13지토요추추체감염결핵균후,국부반응교명현,전신반응교경,완성실험；3지미감염결핵간균.재감염적대백토중,2지출현소수、염식등증상；술후1개월X선양성4지,술후2개월X선양성8지、CT양성12지、MRI양성13지；감염대백토적추체、추방연조직절편소목소-이홍(HE)염색시유농세포、류상피양세포혹배사조등형성,대형성적농종진행농액배양현시69.2％결핵분지간균생장.건립모형성공솔위69.2％.결론 통과재토척주추체국부진행괄당제량H37 Rv결핵표준균주적충식감염,가구건출여인류척주결핵병리변화상사적토척주결핵.
Objective Drill a hole in the lumbar vertebral of the rabbit and implant human type H37 Rv tuberculosis standard strain in the hole to construct the model of spinal tuberculosis of the rabbit.Methods Drill holes at the top of the 5th anodic lumbar vertebrae in 16 rabbits,and fill the holes with gelatin sponge to adsorb the M.tuberculosis suspension 0.1 ml ( the amount of bacteria:5 g/L).Finally,close the holes with bone wax.After operation,adopt examinations of general observation,Imaging,Histopathology,Bacteriology etc at different time periods.Results 13 rabbits whose lumbar vertebral bodies were infected had obvious partial response and lesser response of whole bodies.3 of the 16 rabbits were not infected by mycobacterium tuberculosis.2 of the infected ones appeared to have weight loss,anorexia and other symptoms.One month after operation,4 rabbits' X-ray findings were positive; 4 months after operation,8 rabbits' X-ray findings were positive,12 rabbits' CT findings were realized as positive and 13 rabbits' MRI findings were realized as positive; From the vertebral body and paravertebral soft tissue sections of the infected rabbits we can see the formation of pus cells,epithelioid cells or necrosis.Purulent Juice Culture on abscesses shows that 69.2％ of mycobacterium tuberculosis grew.The success rate of Modeling is 69.2％.Conclusion By implanting proper dosage of human type H37Rv tuberculosis standard strain in the local lumbar vertebral of the rabbit,we can build spinal tuberculosis model of rabbit which is similar to the human spinal TB management changes.