CAJ | 학술논문

目的比较3种HIV抗体确证试剂盒检测HIV早期感染的性能.方法对5份HIV抗体阳性血浆样品进行10倍系列稀释,然后用ELISA检测.对检测结果呈阳性反应的稀释样品分别用3种HIV抗体确证试剂盒进行检测以测试其灵敏度,所用试剂盒包括北京万泰公司的HIV 1+2型抗体检测试剂盒(万泰RIBA)、新加坡MP公司的HIV 1+2型抗体检测试剂盒(MP-WB)和比利时Innogenetics公司的INNO-LIA TM HIV Ⅰ/ⅡScore(INNO-LIA).用这些试剂盒检测11套HIV抗体阳转血清盘中ELISA试验呈阳性反应的样品(共48份).结果对HIV抗体阳性稀释样品的检测结果显示,当5份样品在稀释100倍时,万泰RIBA均检测出阳性结果.在ELISA试验呈阳性反应的48份HIV抗体阳转血清盘样品中,万泰RIBA、MP-WB和INNO-LIA的确证阳性率分别为97.92%(47/48)、81.25%(39/48)和91.67%(44/48).万泰RIBA与MP-WB之间差异有统计学意义(χ2=6.13,P＜0.05),INNO-LIA与MP-WB之间差异有统计学意义(χ2=5.48,P＜0.05),而万泰RIBA与INNO-LIA之间差异无统计学意义(χ2=1.33,P＞0.05).对于含有HIV抗体检测结果不确定样品的6套阳转血清盘,用万泰RIBA、MP-WB和INNO-LIA检测的平均阳转时间分别为0.7、13.3、3.7 d.结论与我国目前常用的MP-WB相比,万泰RIBA和INNO-LIA可以缩短HIV抗体确证的窗口期.
목적 비교3충HIV항체학증시제합검측HIV조기감염적성능.방법 대5빈HIV항체양성혈장양품진행10배계렬희석,연후용ELISA검측.대검측결과정양성반응적희석양품분별용3충HIV항체학증시제합진행검측이측시기령민도,소용시제합포괄북경만태공사적HIV 1+2형항체검측시제합(만태RIBA)、신가파MP공사적HIV 1+2형항체검측시제합(MP-WB)화비리시Innogenetics공사적INNO-LIA TM HIV Ⅰ/ⅡScore(INNO-LIA).용저사시제합검측11투HIV항체양전혈청반중ELISA시험정양성반응적양품(공48빈).결과 대HIV항체양성희석양품적검측결과현시,당5빈양품재희석100배시,만태RIBA균검측출양성결과.재ELISA시험정양성반응적48빈HIV항체양전혈청반양품중,만태RIBA、MP-WB화INNO-LIA적학증양성솔분별위97.92%(47/48)、81.25%(39/48)화91.67%(44/48).만태RIBA여MP-WB지간차이유통계학의의(χ2=6.13,P＜0.05),INNO-LIA여MP-WB지간차이유통계학의의(χ2=5.48,P＜0.05),이만태RIBA여INNO-LIA지간차이무통계학의의(χ2=1.33,P＞0.05).대우함유HIV항체검측결과불학정양품적6투양전혈청반,용만태RIBA、MP-WB화INNO-LIA검측적평균양전시간분별위0.7、13.3、3.7 d.결론 여아국목전상용적MP-WB상비,만태RIBA화INNO-LIA가이축단HIV항체학증적창구기.
Objective This study was to compare the performance of three HIV antibody confirmatory assay kits in confirming early HIV infection. Methods Five HIV antibody-positive plasma specimens were ten-fold serially diluted and then detected by ELISA. The above diluted specimens were detected with the following three HIV antibody coufirmatory assay kits to analyze their sensitivity, including Wantai-RIBA ( Recombinant immunoblot assay, Beijing Wantai Biological Pharmacy, China), MP-WB ( HIV Blot 2. 2 WB,MP Biomedicals Asia Pacific Pte. Ltd. ,Singapore) and INNO-LIA ( INNO-LIATM HIV Ⅰ/Ⅱ Score, Innogenetics N. V. , Belgium), respectively. These kits were further used to detect 48 ELISA-reactive specimens from 11 sets of HIV seroconversion specimens (a total of 48 sanples ) which were previously detected as HIV antibody-positive by ELISA. Results When 5 samples were diluted to 100 fold,Wantai-RIBA still can detect them positive. Among the 48 HIV antibody-positive specimens detected with ELISA,the confirmation positive rate for Wantai-RIBA, MP-WB and INNO-LIA were 97.92% (47/48),81.25 % ( 39/48 ) and 91.67% ( 44/48 ), respectively. There was statistically significant difference between the confirmatory results of Wantai-RIBA and MP-WB ( χ2 = 6. 13, P ＜ 0. 05 ), as well as between those of INNO-LIA and MP-WB ( χ2 = 5.48, P ＜ 0. 05 ); however, there was no statistically significant difference between those of Wantai-RIBA and INNO-LIA ( χ2 = 1.33, P ＞ 0. 05 ). For other six HIV seroconversion panels containing indeterminate specimens, the average seroconversion period of time for Wantai-RIBA,MP-WB and INNO-LIA were 0. 7,13.3 and 3.7 days, respectively. Conclusion Compared with MP-WB,Wantai-RIBA and INNO-LIA could reduce the window period to confirm early HIV infection.