CAJ | 학술논문

目的研究乙型肝炎病毒(HBV)引起的肝炎肝硬化和急性乙型肝炎患者外周血自然杀伤细胞(NK)和活化NK细胞数量的变化及其与HLA-Cw基因型的关系.方法选择肝炎肝硬化和急性乙型肝炎发病期患者各30例及健康对照者41例,采用流式细胞仪检测外周血NK细胞和活化NK细胞的数量,并通过PCR-SSO(polymerase chain reaction-sequence specific oligonucleotide)的方法进行HLA-Cw的基因分型.结果肝炎肝硬化组NK细胞和活化NK细胞的数量分别是13.22%±4.61%和45.68%±14.64%,均低于健康对照组(P<0.05),急性乙型肝炎组NK细胞和活化NK细胞的数量分别是22.62%±3.70%和65.28%±14.45%,均较健康对照组增高(P<0.05),且肝炎肝硬化组与急性乙型肝炎组比较差异有统计学意义(P<0.01);HLA-Cw*15在肝炎肝硬化组中基因频率明显高于健康对照组(P<0.05),且与活化NK细胞数量呈显著负相关(r=-0.862,P<0.05),急性乙型肝炎组与健康对照组间HLA-Cw位点各等位基因的基因型差异无统计学意义(P>0.05).结论肝炎肝硬化患者NK细胞功能低下,HLA-Cw*15基因型可能是通过影响NK细胞而导致HBV感染持续存在的原因之一.
목적 연구을형간염병독(HBV)인기적간염간경화화급성을형간염환자외주혈자연살상세포(NK)화활화NK세포수량적변화급기여HLA-Cw기인형적관계.방법 선택간염간경화화급성을형간염발병기환자각30례급건강대조자41례,채용류식세포의검측외주혈NK세포화활화NK세포적수량,병통과PCR-SSO(polymerase chain reaction-sequence specific oligonucleotide)적방법진행HLA-Cw적기인분형.결과 간염간경화조NK세포화활화NK세포적수량분별시13.22%±4.61%화45.68%±14.64%,균저우건강대조조(P<0.05),급성을형간염조NK세포화활화NK세포적수량분별시22.62%±3.70%화65.28%±14.45%,균교건강대조조증고(P<0.05),차간염간경화조여급성을형간염조비교차이유통계학의의(P<0.01);HLA-Cw*15재간염간경화조중기인빈솔명현고우건강대조조(P<0.05),차여활화NK세포수량정현저부상관(r=-0.862,P<0.05),급성을형간염조여건강대조조간HLA-Cw위점각등위기인적기인형차이무통계학의의(P>0.05).결론 간염간경화환자NK세포공능저하,HLA-Cw*15기인형가능시통과영향NK세포이도치HBV감염지속존재적원인지일.
Objective To investigate the relationship between the frequency of peripheral blood natural killer cells (NK) and HLA-Cw alleles in liver cirrhotic patients with chronic HBV infection and a-cute hepatitis B patients. Methods Thirty liver cirrhotic patients and 30 patients with acute hepatitis B were included in our study, and 41 healthy individuals were enrolled as controls. The numbers of circulating NK cells and activated NK ceils were analyzed by flow cytometry. HLA-Cw genotyping was conducted with polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO). Results The numbers of circu-lating NK cells and activated NK cells in liver cirrhotic patients were 13.22% ± 4.61% and 45.68% ± 14.64%, which was lower than that in healthy subjects (P < 0.05). The numbers of circulating NK cells and activated NK cells in acute hepatitis B patients were 22.62% ± 3.70% and 65.28%± 14.45%, which was higher than that in healthy subjects(P < 0. 05). There were statistically significant differences between the two groups(P < 0.01). The allele frequency of HLA-Cw * 15 in the patients with cirrhosis was signifi-cantly higher than that in the healthy (P < 0.05), and there was a significant negative correlation between the frequency of HLA-Cw * 15 and the numbers of activated NK cells in liver cirrhosis(r =4). 862, P < 0.05). No statistically significance was found between the group of acute hepatitis B and healthy subjects a- bout HLA-Cw(P > 0. 05). Conclusion The function of NK cells in liver cirrhotic patients is low, HLA-Cw * 15 gene may be one of the causes of effecting the antiviral function of NK ceils to induce the persistence of hepatitis B virus(HBV) infection.