CAJ | 학술논문

在最近完成测序的水稻籼稻和粳稻两个亚种基因组中,各找到564和519个较为可靠的tRNA基因,进一步证实了于2002年发表的基于基因组序列草图的分析结果.修正的摆动假设,即至少需要46种tRNA基因才能译出61种可能的反密码子,在这两个亚种中均准确成立.在这46种tRNA中,有些在籼稻和粳稻中的序列均全同.有18种水稻tRNA与拟南芥中的相应序列全同.在籼稻基因组序列中还发现了384个5S rRNA基因,一批17S 和5.8S rRNA基因以及一个 25S rRNA基因.这些rRNA基因的不完备是由于它们通常以串接阵列形式存在于异染色质区域,而后者在全基因组霰弹法测序中不易完整测出.在tRNA和rRNA基因序列之间发现了多处互补片段,这将有助于研究它们的进化和相互作用.
재최근완성측서적수도선도화갱도량개아충기인조중,각조도564화519개교위가고적tRNA기인,진일보증실료우2002년발표적기우기인조서렬초도적분석결과.수정적파동가설,즉지소수요46충tRNA기인재능역출61충가능적반밀마자,재저량개아충중균준학성립.재저46충tRNA중,유사재선도화갱도중적서렬균전동.유18충수도tRNA여의남개중적상응서렬전동.재선도기인조서렬중환발현료384개5S rRNA기인,일비17S 화5.8S rRNA기인이급일개 25S rRNA기인.저사rRNA기인적불완비시유우타문통상이천접진렬형식존재우이염색질구역,이후자재전기인조산탄법측서중불역완정측출.재tRNA화rRNA기인서렬지간발현료다처호보편단,저장유조우연구타문적진화화상호작용.
In the recently assembled genomes of rice Oryza sativa ssp.indica and japonica,we identified 564 and 519 tRNA genes,respectively.The modified wobble hypothesis,namely,at least 46 tRNA species must present in order to decode all 61 possible anticodons,is perfectly observed in both subspecies.Among the 46 tRNA species,indica and japonica have many identical ones in sequence.There are 18 rice tRNA species that have identical counterparts in Arabidopsis.In the indica superscaffold dataset,384 5S rRNA genes,dozens of 17S and 5.8S rRNA genes and one 25S rRNA gene were discovered.The incompleteness of observed rRNA genes is mainly caused by the fact that the rRNA genes always exist as tandem arrays in heterochromatic regions that are not successfully sequenced in a whole-genome shotgun approach.