CAJ | 학술논문

目的:探讨增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)在小鼠胚胎着床过程中的作用.方法:实时荧光定量聚合酶链反应(Real-time Fluorescence Quantitative PCR,Real-time FQ-PCR)技术检测未孕及孕2、3、4、5、6、7 d(分别记为d0、d2、d3、d4、d5、d6、d7)小鼠子宫内膜PCNA mRNA的表达;子宫角注射PCNA单克隆抗体观察胚泡着床数.结果:FQ-PCR测得随着小鼠妊娠天数的增加,PCNA mRNA表达量也逐渐增高,在孕d4、d5达到峰值,孕d6、d7逐渐下降.子宫角注射PCNA单克隆抗体后胚泡着床数明显减少.结论:PCNA在妊娠早期子宫内膜持续表达,可能参与了胚泡着床.
목적:탐토증식세포핵항원(Proliferating cell nuclear antigen,PCNA)재소서배태착상과정중적작용.방법:실시형광정량취합매련반응(Real-time Fluorescence Quantitative PCR,Real-time FQ-PCR)기술검측미잉급잉2、3、4、5、6、7 d(분별기위d0、d2、d3、d4、d5、d6、d7)소서자궁내막PCNA mRNA적표체;자궁각주사PCNA단극륭항체관찰배포착상수.결과:FQ-PCR측득수착소서임신천수적증가,PCNA mRNA표체량야축점증고,재잉d4、d5체도봉치,잉d6、d7축점하강.자궁각주사PCNA단극륭항체후배포착상수명현감소.결론:PCNA재임신조기자궁내막지속표체,가능삼여료배포착상.
Objective:To investigate the effect of PCNA in the mouse uterus during embryo implantation.Methods:Real-time fluorescence quantitative PCR (FQ -PCR) was used to detect the PCNA mRNA of the mouse of unpregnancy, pregnancy at d2, d3, d4, d5, d6 and d7. PCNA monoclonal antibody was injected in horn of uterus at pregnant d3. Results: The expression of PCNA mRNA showed gradual hoist trend as days passed , and at pregnant d4, d5 it reached the maximum, at pregnant d6,d7 it showed gradual descent trend. The number of blastocysts in horn of uterus which was injected PCNA monoclonal antibody was smaller than in that of injected with Saline. Conclusion: PCNA is persistently expressed in mouse endometrium during the early pregnancy and maybe participates in the regulation process of mouse blastodysts implantation .