湖北农业科学
湖北農業科學
호북농업과학
2010年
1期
5-9
,共5页
砷%亚砷酸氧化菌%亚砷酸氧化酶基因%反向PCR%Sinorhizobium
砷%亞砷痠氧化菌%亞砷痠氧化酶基因%反嚮PCR%Sinorhizobium
신%아신산양화균%아신산양화매기인%반향PCR%Sinorhizobium
arsenic%arsenite-oxidizing bacterium%arsenite oxidase gene%reverse PCR%Sinorhizobium
亚砷酸氧化细菌能够将毒性大的As(Ⅲ)氧化成毒性小的As(V),在生物修复砷污染方面具有应用价值.对一株新型亚砷酸氧化菌Sinorhizobium sp.GW3进行了较全面的鉴定,并分离及分析了亚砷酸氧化酶基因aoxAB.形态学、生理生化鉴定和16S rRNA基因等分析结果表明该菌为Sinorhizobium属.该菌对As(Ⅲ)抗性的MIC(Minimum inhibitory concentration)为9 mmol/L.首次在Sinorhizobium属中分离了包括编码小亚基aoxA和大亚基aoxB在内的亚砷酸氧化酶基因,其编码的大小亚基与已发现的Agrobacterium tumefaciens(ABB51929)的大小亚基在氨基酸水平上分别有86%、80%的同源性.
亞砷痠氧化細菌能夠將毒性大的As(Ⅲ)氧化成毒性小的As(V),在生物脩複砷汙染方麵具有應用價值.對一株新型亞砷痠氧化菌Sinorhizobium sp.GW3進行瞭較全麵的鑒定,併分離及分析瞭亞砷痠氧化酶基因aoxAB.形態學、生理生化鑒定和16S rRNA基因等分析結果錶明該菌為Sinorhizobium屬.該菌對As(Ⅲ)抗性的MIC(Minimum inhibitory concentration)為9 mmol/L.首次在Sinorhizobium屬中分離瞭包括編碼小亞基aoxA和大亞基aoxB在內的亞砷痠氧化酶基因,其編碼的大小亞基與已髮現的Agrobacterium tumefaciens(ABB51929)的大小亞基在氨基痠水平上分彆有86%、80%的同源性.
아신산양화세균능구장독성대적As(Ⅲ)양화성독성소적As(V),재생물수복신오염방면구유응용개치.대일주신형아신산양화균Sinorhizobium sp.GW3진행료교전면적감정,병분리급분석료아신산양화매기인aoxAB.형태학、생리생화감정화16S rRNA기인등분석결과표명해균위Sinorhizobium속.해균대As(Ⅲ)항성적MIC(Minimum inhibitory concentration)위9 mmol/L.수차재Sinorhizobium속중분리료포괄편마소아기aoxA화대아기aoxB재내적아신산양화매기인,기편마적대소아기여이발현적Agrobacterium tumefaciens(ABB51929)적대소아기재안기산수평상분별유86%、80%적동원성.
In this stuay,a novel arsenite-oxidizing bacterium GW3 was identified and its arsenite oxidase gene aoxAB was isolated.Based on the morphological, physiological/biochemical characteristics and 16S rRNA gene sequence analyses,this strain was identified as Sinorhizobium sp.GW3.The MIC(Minimum Inhibitory Concentration)for arsenite was 9 mmol/L.This was the first report to isolate full length arsenite oxidase gene including aoxA and aoxB from Sinorhizobium.The aoxA and aoxB of Sinorhizobium sp.GW3 showed 80%and 86%amino acid identifies with the aoxA and aoxB of Agrobacterium tumefaciens(ABB51929),respectively.
