CAJ | 학술논문

将被金龟子绿僵菌MaYTTR-04菌株感染后的松墨天牛成虫僵虫放置于林地中,研究在林间自然条件下僵成虫体上绿僵菌孢子的宿存动态.通过2年试验发现:林间松墨天牛僵成虫体上孢子数量在试验初期(42天内)显著下降,以后下降趋势趋缓,84天后至僵虫完全腐烂基本稳定在每成虫10~5个孢子数量级;林地僵虫体上的孢子在126天内萌发率均保持在90%以上的较高水平.通过对不同时间僵虫体上绿僵菌对松墨天牛成虫的致病力研究发现,在10~7,10~6孢子·mL~(-1)数量级的接种量下,僵成虫体上的孢子致病力与对照差异不显著,说明僵虫体上的孢子在野外宿存的过程中可以较好地保持其致病力.
장피금구자록강균MaYTTR-04균주감염후적송묵천우성충강충방치우임지중,연구재림간자연조건하강성충체상록강균포자적숙존동태.통과2년시험발현:림간송묵천우강성충체상포자수량재시험초기(42천내)현저하강,이후하강추세추완,84천후지강충완전부란기본은정재매성충10~5개포자수량급;임지강충체상적포자재126천내맹발솔균보지재90%이상적교고수평.통과대불동시간강충체상록강균대송묵천우성충적치병력연구발현,재10~7,10~6포자·mL~(-1)수량급적접충량하,강성충체상적포자치병력여대조차이불현저,설명강충체상적포자재야외숙존적과정중가이교호지보지기치병력.
Monochamus alternatus is a major insect vector transmitting pinewood nematode disease, an important quarantine disease of pine forests. In this paper, the muscardine cadavers of M. altematus adults died by Metarhizium anisopliae were put in the forest floor,and the dynamics of spores on the cadaver in natural status was studied. The knowledge would provide a theoretic basis for M. alternatus's biocontrolling using M. anisopliae as an agent. In the two-years experiment, the amount of spores on the cadaver decreased significantly in the first 42 days, and then decreased slightly. The amount of spores on the cadaver was maintained at the level of 10~5 spores per adult from the 84th days to the end of the experiment. Spore germination rates were always above 90% within 126 days. Then the virulence of M.anisopliae to M. alternatus was tested in different time, and the difference of the virulence between the spores and the control was not significant when M. alternatus inoculated with 10~7 or 10~6 spores·mL~(-1).The results showed that spores on the cadaver could keep high virulence to M. alternatus for a long time in field.