CAJ | 학술논문

利用瘤胃模拟体外产气法研究在高精料底物条件下添加富含十八碳不饱和脂肪酸的植物油和亚麻酸对瘤胃发酵和甲烷生成的影响.在植物油的筛选试验中,菜籽油、豆油和亚麻油分别添加底物干物质的10%,观察其对甲烷生成和发酵产物的影响.结果表明,豆油和亚麻油显著降低了产气量和甲烷产量,但甲烷产量的下降幅度更大,菜籽油无效果.同时,豆油和亚麻油显著升高了总挥发性脂肪酸含量和丙酸比例.选取筛选出的效果最显著的植物油--亚麻油,和亚麻酸一起比较研究其对瘤胃发酵的剂量效应,亚麻油和亚麻酸分别添加底物干物质的0%、3%、5%、7%和9%.亚麻油和亚麻酸分别在添加量为5%和3%时显著降低了甲烷产量,且抑制效果随添加剂量的提高而增强.亚麻油和亚麻酸显著改变瘤胃发酵模式向丙酸型发酵转变.这些结果表明,一些富含十八碳不饱和脂肪酸的植物油和亚麻酸可以在高精料日粮条件下改善瘤胃发酵,降低甲烷生成,且植物油抑制甲烷生成的效果与添加剂量和所含脂肪酸的不饱和度有关.
이용류위모의체외산기법연구재고정료저물조건하첨가부함십팔탄불포화지방산적식물유화아마산대류위발효화갑완생성적영향.재식물유적사선시험중,채자유、두유화아마유분별첨가저물간물질적10%,관찰기대갑완생성화발효산물적영향.결과표명,두유화아마유현저강저료산기량화갑완산량,단갑완산량적하강폭도경대,채자유무효과.동시,두유화아마유현저승고료총휘발성지방산함량화병산비례.선취사선출적효과최현저적식물유--아마유,화아마산일기비교연구기대류위발효적제량효응,아마유화아마산분별첨가저물간물질적0%、3%、5%、7%화9%.아마유화아마산분별재첨가량위5%화3%시현저강저료갑완산량,차억제효과수첨가제량적제고이증강.아마유화아마산현저개변류위발효모식향병산형발효전변.저사결과표명,일사부함십팔탄불포화지방산적식물유화아마산가이재고정료일량조건하개선류위발효,강저갑완생성,차식물유억제갑완생성적효과여첨가제량화소함지방산적불포화도유관.
A series of in vitro studies were performed to evaluate the effects of unsaturated C18 fatty acid-rich oils and linolenic acid on rumen fermentation and methanogenesis with a high-grain substrate.In oil screening experiment,rapeseed oil,soybean oil and linseed oil were used as tested additives.Rumen fluid from sheep was incubated with ground corn with or without oil supplementation (10% of substrate DM) for 24 h to monitor rumen products.Gas production and methane emission decreased with addition of unsaturated C18 fatty acid-rich oils except rapeseed oil.Soybean oil and linseed oil increased total volatile fatty acids (VFA) production and molar proportion of propionate.In dose-response experiment,the most potent additives--linseed oil,and free linolenic acid were further tested to determine dose-response of rumen fermentation.Linseed oil and free linolenic acid were added to basal substrate at 0%,3%,5%,7% and 9%,respectively.Linseed oil and linolenic acid decreased methane emission significantly and altered rumen fermentation toward more propionate production by supplementation at 5% and 3%,respectively.These results suggested that some unsaturated C18 fatty acid-rich oils and free linolenic acid could be used for improving rumen fermentation under high-grain diet,and the effect of vegetable oils on methane emission was concerned with the type and inclusion levels.In the present study,methane formation was decreased significantly with increasing levels and unsaturation of fatty acids in vegetable oils.