国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2008年
7期
507-510
,共4页
Zhi-Bo Zhang%陈本阳%Xuan Tang%彭旭%Lu Tang
Zhi-Bo Zhang%陳本暘%Xuan Tang%彭旭%Lu Tang
Zhi-Bo Zhang%진본양%Xuan Tang%팽욱%Lu Tang
参芎注射液%脑缺血%再灌注损伤%凋亡%Fas相关死亡结构域蛋白
參芎註射液%腦缺血%再灌註損傷%凋亡%Fas相關死亡結構域蛋白
삼궁주사액%뇌결혈%재관주손상%조망%Fas상관사망결구역단백
Shertxiong injection%cerebral ischemia%reperfusion injury%apoptosis%Fas-associated death domain protein
目的:探讨参芎注射液对大鼠脑缺血再灌注后神经细胞凋亡及Fas相关死亡结构域蛋白(FADD)及其mRNA表达的影响.方法:100只SD大鼠随机分为正常组(n=10)、假手术组(n=10)、脑缺血再灌注组(n=40)和参芎注射液组(n40).线栓法制备大脑中动脉闭塞模型,TUNEL法检测神经细胞凋亡,分别应用免疫组织化学染色和逆转录-聚合酶链式反应检测FADD蛋白和mRNA表达.结果:与正常组和假手术组相比,脑缺血再灌注组凋亡神经细胞计数显著增加(P<0.01),FADD蛋白和mRNA表达均显著增强(P均<0.01).与脑缺血再灌注组比较,参芎注射液组凋亡神经细胞显著减少(P<0.05,P<0.01),FADD蛋白和mRNA表达显著减弱(P<0.05,P<0.01).结论:参芎注射液能通过下调FADD表达抑制大鼠脑缺血再灌注后神经细胞凋亡.
目的:探討參芎註射液對大鼠腦缺血再灌註後神經細胞凋亡及Fas相關死亡結構域蛋白(FADD)及其mRNA錶達的影響.方法:100隻SD大鼠隨機分為正常組(n=10)、假手術組(n=10)、腦缺血再灌註組(n=40)和參芎註射液組(n40).線栓法製備大腦中動脈閉塞模型,TUNEL法檢測神經細胞凋亡,分彆應用免疫組織化學染色和逆轉錄-聚閤酶鏈式反應檢測FADD蛋白和mRNA錶達.結果:與正常組和假手術組相比,腦缺血再灌註組凋亡神經細胞計數顯著增加(P<0.01),FADD蛋白和mRNA錶達均顯著增彊(P均<0.01).與腦缺血再灌註組比較,參芎註射液組凋亡神經細胞顯著減少(P<0.05,P<0.01),FADD蛋白和mRNA錶達顯著減弱(P<0.05,P<0.01).結論:參芎註射液能通過下調FADD錶達抑製大鼠腦缺血再灌註後神經細胞凋亡.
목적:탐토삼궁주사액대대서뇌결혈재관주후신경세포조망급Fas상관사망결구역단백(FADD)급기mRNA표체적영향.방법:100지SD대서수궤분위정상조(n=10)、가수술조(n=10)、뇌결혈재관주조(n=40)화삼궁주사액조(n40).선전법제비대뇌중동맥폐새모형,TUNEL법검측신경세포조망,분별응용면역조직화학염색화역전록-취합매련식반응검측FADD단백화mRNA표체.결과:여정상조화가수술조상비,뇌결혈재관주조조망신경세포계수현저증가(P<0.01),FADD단백화mRNA표체균현저증강(P균<0.01).여뇌결혈재관주조비교,삼궁주사액조조망신경세포현저감소(P<0.05,P<0.01),FADD단백화mRNA표체현저감약(P<0.05,P<0.01).결론:삼궁주사액능통과하조FADD표체억제대서뇌결혈재관주후신경세포조망.
Objective:To imestigate the effect of Shenxiong injection on neuronal aooptosis and Fasassociated death dormin protein(FADD)and its mRNA expression after ischernia-reperfusion in rats.Methods:Atotal of 100 SD rats wgre randomly allocated into normal(n=10),sham-operation(n=10),cerebral ischemia-reperfusion(n=40),and Shenxiong injection(n=40)groups.A model of middle cerebral wtery occlusion was induced by suture method.The neuronal apoptosis was detected by the TUNEL assay.The expressions of FADD protein and mRNA were detected by inmmnohistochemical staining and reverse tramcription-polymerase chain reaction(RT-PCR),respectively.Results:As compared with the normal and sham-operation groups.the numbers of apoptotic cell in the cerebral ischemia-reperfusion group were increased significantly(P<0.01),and the expressions of FADD protein and mRNA were enhanced significantly(all P<0.01).As compared with the ceretral ischemia-reperfusion group,the numbers of apoptotic cell were decreased significantly (P<0.05,P<0.001),and the expressiom of FADD protein and mRNA were reduced significantly in the Shenxiong group(P<0.05,P<o.001).Convlusions:Shertxiong injection may inhibit the neuronal apoptosis after ischemia-reprfusion in r-cas by down-regulaftng the expression of FADD.