CAJ | 학술논문

目的了解目前广东省HIV/AIDS患者HIV-1基因型的流行状况.方法用套式PCR对222例HIV/AIDS患者外周血单核细胞(PBMC)中HIV前病毒DNA膜蛋白env基因C2-V4区及其邻近区域片段进行扩增,并对片段进行序列测定,与国际HIV-1标准毒株序列进行比较,依据同源性确定基因型.结果 222例患者中,111例PCR扩增阳性并得到相应的序列,66例(59-46%)为CRF01-AE重组亚型,18例(16.22%)为BC型,18例(16.22%)为B亚型,9例(8.11%)为C亚型.不同性别、不同年龄组、不同感染来源的HIV-1感染均以CRF01-AE为主(均为50%以上).对抗逆转录病毒治疗12个月临床有效的57例患者,CD4+T细胞计数的变化在各基因型分组及各治疗方案分组间的差异无统计学意义(F=95.72、85.61,P＞0.05).结论广东省HIV/AIDS患者中,目前流行的HIV-1毒株以CRF01-AE亚型为主,同时有BC(包括CRF07-BC、08-BC重组亚型)及B、C亚型存在,基因型分布与性别、年龄无明显相关性,基因型与抗逆转录病毒治疗方案的选择、疗效均无相关性.
목적 료해목전광동성HIV/AIDS환자HIV-1기인형적류행상황.방법 용투식PCR대222례HIV/AIDS환자외주혈단핵세포(PBMC)중HIV전병독DNA막단백env기인C2-V4구급기린근구역편단진행확증,병대편단진행서렬측정,여국제HIV-1표준독주서렬진행비교,의거동원성학정기인형.결과 222례환자중,111례PCR확증양성병득도상응적서렬,66례(59-46%)위CRF01-AE중조아형,18례(16.22%)위BC형,18례(16.22%)위B아형,9례(8.11%)위C아형.불동성별、불동년령조、불동감염래원적HIV-1감염균이CRF01-AE위주(균위50%이상).대항역전록병독치료12개월림상유효적57례환자,CD4+T세포계수적변화재각기인형분조급각치료방안분조간적차이무통계학의의(F=95.72、85.61,P＞0.05).결론 광동성HIV/AIDS환자중,목전류행적HIV-1독주이CRF01-AE아형위주,동시유BC(포괄CRF07-BC、08-BC중조아형)급B、C아형존재,기인형분포여성별、년령무명현상관성,기인형여항역전록병독치료방안적선택、료효균무상관성.
Objective To explore the current status of HIV-1 genotype distribution in patients with HIV/AIDS in Guangdong province.Methods HIV-1 pro-viral DNA was extracted from PBMC,and env C2-V4 gene was amplified by PCR from 222 cases.The PCR product was subject to being sequenced.The acquired gene sequence was compared with that of standard strains to identify the genotype.Results HIV-1 genotype was successfully identified in 111 HIV/AIDS patients.66 patients(59.46%) had recombinant genotype CRF01-AE infection,18(16.22%) had recombinant BC type infection,and 18(16.22%)had subtype B,9(8.11%) had subtype C infection.CRF01-AE was predominant in all the patients with different gender,age and transmission route.All the 57 patients with AIDS who received highly antiretroviral therapy(HAART) for more than 12 months had good clinical response to the treatment.The differences of CD4+ cell counts among either the subtype groups or HAART regime groups were not obviously( F = 95.72,85.61,P＞0.05).Conclusions The current predominant circulated HIV-1 genotype is CRF01-AE in Guangdong province.Other genotypes include subtype BC(including CRF07-BC and CRF08-BC),subtype B and subtype C.The HIV-1 subtypes have no association with gender,age,the antiretroviral regimens or the effectiveness of treatment.