中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2011年
10期
848-853
,共6页
董怿%周兵%牛延涛%王振常
董懌%週兵%牛延濤%王振常
동역%주병%우연도%왕진상
疾病模型,动物%鼻窦炎%骨重建%体层摄影术,X线计算机
疾病模型,動物%鼻竇炎%骨重建%體層攝影術,X線計算機
질병모형,동물%비두염%골중건%체층섭영술,X선계산궤
Disease models,animal%Sinusitis%Bone remodeling%Tomography,X-ray computed
目的 采用CT扫描记录和分析兔鼻窦炎模型造模后不同时间及给药后骨质重塑,探讨测量模型窦壁骨质CT值可否评价骨质重塑的程度以及经糖皮质激素治疗的模型骨质重塑程度是否减轻.方法 将48只新西兰白兔上颌窦内接种金黄色葡萄球菌,制成兔鼻窦炎模型.根据造模时间将兔分为3组:A组(造模后4周)、B组(造模后8周)、C组(造模后12周),每组16只；每组再分为给药和对照2个亚组,每个亚组8只.造模前所有兔行鼻窦CT检查排除鼻腔、鼻窦病变并以此CT图像作为阴性对照.3个组的对照亚组造模后不做任何处理,到达实验周数时行鼻窦CT检查；3个组的给药亚组到达实验周数的前2周开始注射地塞米松磷酸钠注射液,给药2周后行鼻窦CT检查.分别测量显示上颌窦骨壁的各幅CT图像中骨质最厚处的CT值.以SPSS 16.0软件对CT值进行统计学分析,比较造模后不同时间及是否给药的兔鼻窦炎模型骨质重塑情况.结果 造模前正常兔鼻窦骨壁的CT值平均值((x)±s,下同)为810.0±99.7,造模后CT值平均值:A组对照组为964.0±84.6、给药组为833.0±92.5；B组对照组为987.0±91.5、给药组为886.0 ±91.6;C组对照组为1086.0±74.0、给药组为899.8±76.5.A、B、C组造模后CT值均高于造模前(t值分别为2.747、4.513、7.350,P值均＜0.05).A组和B组对照组CT值比较差异无统计学意义(t=0.423,P=0.677)；C组对照组CT值高于B组(t=3.905,P=0.001)；A组和B组给药组CT值比较差异无统计学意义(t =0.892,P=0.384)；B组和C组给药组的CT值比较差异无统计学意义(t=0.886,P=0.385).A、B、C组给药组CT值均低于对照组(t值分别为2.717、3.687、8.379,P值均＜0.05).结论 兔鼻窦炎模型中,鼻窦窦壁骨质重塑现象普遍存在,并随造模时间延长而更明显.CT值测量可反映骨质重塑程度.糖皮质激素对兔鼻窦炎模型骨质重塑现象有抑制作用.
目的 採用CT掃描記錄和分析兔鼻竇炎模型造模後不同時間及給藥後骨質重塑,探討測量模型竇壁骨質CT值可否評價骨質重塑的程度以及經糖皮質激素治療的模型骨質重塑程度是否減輕.方法 將48隻新西蘭白兔上頜竇內接種金黃色葡萄毬菌,製成兔鼻竇炎模型.根據造模時間將兔分為3組:A組(造模後4週)、B組(造模後8週)、C組(造模後12週),每組16隻；每組再分為給藥和對照2箇亞組,每箇亞組8隻.造模前所有兔行鼻竇CT檢查排除鼻腔、鼻竇病變併以此CT圖像作為陰性對照.3箇組的對照亞組造模後不做任何處理,到達實驗週數時行鼻竇CT檢查；3箇組的給藥亞組到達實驗週數的前2週開始註射地塞米鬆燐痠鈉註射液,給藥2週後行鼻竇CT檢查.分彆測量顯示上頜竇骨壁的各幅CT圖像中骨質最厚處的CT值.以SPSS 16.0軟件對CT值進行統計學分析,比較造模後不同時間及是否給藥的兔鼻竇炎模型骨質重塑情況.結果 造模前正常兔鼻竇骨壁的CT值平均值((x)±s,下同)為810.0±99.7,造模後CT值平均值:A組對照組為964.0±84.6、給藥組為833.0±92.5；B組對照組為987.0±91.5、給藥組為886.0 ±91.6;C組對照組為1086.0±74.0、給藥組為899.8±76.5.A、B、C組造模後CT值均高于造模前(t值分彆為2.747、4.513、7.350,P值均＜0.05).A組和B組對照組CT值比較差異無統計學意義(t=0.423,P=0.677)；C組對照組CT值高于B組(t=3.905,P=0.001)；A組和B組給藥組CT值比較差異無統計學意義(t =0.892,P=0.384)；B組和C組給藥組的CT值比較差異無統計學意義(t=0.886,P=0.385).A、B、C組給藥組CT值均低于對照組(t值分彆為2.717、3.687、8.379,P值均＜0.05).結論 兔鼻竇炎模型中,鼻竇竇壁骨質重塑現象普遍存在,併隨造模時間延長而更明顯.CT值測量可反映骨質重塑程度.糖皮質激素對兔鼻竇炎模型骨質重塑現象有抑製作用.
목적 채용CT소묘기록화분석토비두염모형조모후불동시간급급약후골질중소,탐토측량모형두벽골질CT치가부평개골질중소적정도이급경당피질격소치료적모형골질중소정도시부감경.방법 장48지신서란백토상합두내접충금황색포도구균,제성토비두염모형.근거조모시간장토분위3조:A조(조모후4주)、B조(조모후8주)、C조(조모후12주),매조16지；매조재분위급약화대조2개아조,매개아조8지.조모전소유토행비두CT검사배제비강、비두병변병이차CT도상작위음성대조.3개조적대조아조조모후불주임하처리,도체실험주수시행비두CT검사；3개조적급약아조도체실험주수적전2주개시주사지새미송린산납주사액,급약2주후행비두CT검사.분별측량현시상합두골벽적각폭CT도상중골질최후처적CT치.이SPSS 16.0연건대CT치진행통계학분석,비교조모후불동시간급시부급약적토비두염모형골질중소정황.결과 조모전정상토비두골벽적CT치평균치((x)±s,하동)위810.0±99.7,조모후CT치평균치:A조대조조위964.0±84.6、급약조위833.0±92.5；B조대조조위987.0±91.5、급약조위886.0 ±91.6;C조대조조위1086.0±74.0、급약조위899.8±76.5.A、B、C조조모후CT치균고우조모전(t치분별위2.747、4.513、7.350,P치균＜0.05).A조화B조대조조CT치비교차이무통계학의의(t=0.423,P=0.677)；C조대조조CT치고우B조(t=3.905,P=0.001)；A조화B조급약조CT치비교차이무통계학의의(t =0.892,P=0.384)；B조화C조급약조적CT치비교차이무통계학의의(t=0.886,P=0.385).A、B、C조급약조CT치균저우대조조(t치분별위2.717、3.687、8.379,P치균＜0.05).결론 토비두염모형중,비두두벽골질중소현상보편존재,병수조모시간연장이경명현.CT치측량가반영골질중소정도.당피질격소대토비두염모형골질중소현상유억제작용.
Objective To evaluate the findings of computed tomography (CT) as objective markers of bone remodeling in rabbit models with chronic rhinosinusitis.Methods Forty-eight rabbit models were 1established by vaccination of staphylococcus aureus.The rabbits were divided into 3 groups according to the time of infection:group A,B and C (4,8,12 weeks after infection),16 rabbits in each group.Each group was subdivided into the medication administration team and the control team,8 rabbits in each team.All the rabbits were examined by CT before vaccination to rule out the disease of nasal cavity and sinuses,and the CT images were used as the negative control.No interference was given to the control teams which were only examined again by CT when reached the end week.Dexamethasone sodium phosphate were administered to the medication administration teams 2 weeks before the end of experiment,and were examined by CT at the end.The images of both horizontal position and coronal position by reconstruction were obtained.The Hounsfield unit (Hu) of the bone which was the thickest position in each image were measured.The data was analyzed by SPSS 16.0 software.The Hu was analyzed statistically to compare the situation of the bone remodeling in different periods and administration in the rabbit models with CRS.Results Average Hu ((x) ±s) of normal rabbits was 810.0 ± 99.7,average Hu at the end time:control team in group A was 964.0 ± 84.6,medication administration team in group A was 833.0 ± 92.5 ; control team in group B was 987.0 ± 91.5,medication administration team in group B was 886.0 ± 91.6; control team in group C was 1086.0 ± 74.0,medication administration team in group C was 899.8 ± 76.5.The Hu in all groups were higher than normal (t value were 2.747,4.5 1 3 and 7.350 respectively,all P ＜ 0.05 ).No statistical difference was found between control teams of group A and B ( t =0.423,P =0.667 ).The Hu in control team of group C was higher than group B ( t =3.905,P =0.001 ) ; There was no statistical difference between medication administration teams of group A and B ( t =0.892,P =0.384 ),and group B and C ( t =0.886,P =0.385 ).The Hu of all medication administration teams in 3 groups were lower than all the control teams ( t value were 2.717,3.687,8.379 respectively,all P ＜ 0.05 ).Conclusions Bone remodeling was found in rabbit models with rhinosinusitis,and the phenomenon was more obvious if the period was lengthened.The Hu could reflect the degree of bone remodelling.Glucocorticoids could depress the bone remodeling in the rabbit models with rhinosinusitis.
