CAJ | 학술논문

目的观察在组织工程骨内植入神经束后大段组织工程骨的长期成骨效果.方法新西兰大白兔64只,随机分为四组:A组,单纯组织工程骨组;B组,运动神经束植入组(股神经肌支);C组,感觉神经束植入组(隐神经);D组,感觉、运动神经束联合植入组.每只动物均在左侧股骨制作长1.5 cm的段缺性骨与骨膜缺损,钢板固定后在骨缺损处分别植入四种方法制备的组织工程骨.术后1、3、6、12个月行大体观察、X线和组织学定量观察成骨情况. 结果术后1、3、6个月时,在组织工程骨内植入感觉神经或联合植入两种神经后,比单纯组织工程骨和运动神经束植入的修复效果均有明显提高.术后12个月时,各组骨再生情况基本一致,但新生骨组织出现一定程度的吸收,其外观较正常股骨细,新生骨组织与兔股骨牢固愈合,并开始塑形且出现髓腔再通. 结论在组织工程骨内植入感觉神经可促进成骨,而植入运动神经未见促进作用;组织工程骨可以修复兔大段骨缺损;该实验新生骨组织的吸收可能与模型的制作方法有关.
목적 관찰재조직공정골내식입신경속후대단조직공정골적장기성골효과.방법 신서란대백토64지,수궤분위사조:A조,단순조직공정골조;B조,운동신경속식입조(고신경기지);C조,감각신경속식입조(은신경);D조,감각、운동신경속연합식입조.매지동물균재좌측고골제작장1.5 cm적단결성골여골막결손,강판고정후재골결손처분별식입사충방법제비적조직공정골.술후1、3、6、12개월행대체관찰、X선화조직학정량관찰성골정황. 결과 술후1、3、6개월시,재조직공정골내식입감각신경혹연합식입량충신경후,비단순조직공정골화운동신경속식입적수복효과균유명현제고.술후12개월시,각조골재생정황기본일치,단신생골조직출현일정정도적흡수,기외관교정상고골세,신생골조직여토고골뢰고유합,병개시소형차출현수강재통. 결론 재조직공정골내식입감각신경가촉진성골,이식입운동신경미견촉진작용;조직공정골가이수복토대단골결손;해실험신생골조직적흡수가능여모형적제작방법유관.
Objective To investigate the long-term effects of nerve-tract Implantation on in vivo osteogenesis of large tissue-engineered bone. Methods A segmental bone defect of 1.5 cm long was made at the left femur in each of the 64 New Zealand rabbits which were divided into 4 equal groups randomly.After plate fixation, the defects of each group were implanted with the engineered-bone prepared in each of the following 4 ways respectively: non-nerve implantation (group A), motor nerve-tract implantation (group B,muscular branches of femoral nerves), sensory nerve-tract implantation (group C, saphenous nerves), and sensory plus motor nerve-tract implantation (group D) . At 1, 3, 6 and 12 months postoperatively, gross observation, X-ray examination and histological examination were performed to evaluate the engineered-bone and the new bone formation. Results At 1, 3 and 6 months post-operatively, implantation of sensory nerve into the tissue-engineered bone (groups C and D) bad obviously better reparative effect than the methods of groups A and B. At 12 months post-operatively, there was no significant difference in osteanagenesis among the 4 groups, and gradual absorption of the new forming bone occurred. The new bone formation appeared thinner than the normal femur but bound firmly with the femur. Moulding and medullary cavity reeanalization were observed in the new bone formation. Conclusions Implantation of sensory nerve-tract into tis-sue-engineered bone can improve osteogenesis, but implantation of motor nerve-tract seems to have no such effect. Tissue-engineered bone may repair large bone defects in rabbits.