中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
2期
170-172
,共3页
廖淳杰%唐小曼%覃怡%谢玉波
廖淳傑%唐小曼%覃怡%謝玉波
료순걸%당소만%담이%사옥파
二异丙酚%依托咪酯%海马%细胞凋亡%儿童,学龄前(2-5)
二異丙酚%依託咪酯%海馬%細胞凋亡%兒童,學齡前(2-5)
이이병분%의탁미지%해마%세포조망%인동,학령전(2-5)
Propofol%Etomidate%Hippocampus%Apoptosis%Child pre(2-5)
目的 评价异丙酚或依托咪酯对幼龄大鼠海马神经元凋亡的影响.方法 雄性SD大鼠140只,4周龄,体重40～60 g,采用随机数字表法,将大鼠随机分为7组(n=20):对照组(C组)、异丙酚50 mg/kg组(P1组)、异丙酚100 mg/kg组(P2组)、异丙酚200 mg/kg组(P3组)、依托咪酯10 mg/kg组(E1组)、依托咪酯30 mg/kg组(E2组)和依托咪酯60 mg/kg组(E3组).P1组和E1组单次腹腔注射异丙酚50 mg/kg或依托咪酯10 mg/kg；P2组、P3组、E2组和E3组首次注射异丙酚50 mg/kg或依托咪酯10 mg/kg,待大鼠有体动反应后,再每次追加异丙酚50 mg/kg或依托咪酯10 mg/kg,直至给完总量.大鼠苏醒2h时,每组取5只大鼠,抽取动脉血样进行血气分析.每组取5只大鼠,取海马CA1区,电镜下观察超微结构.每组取5只大鼠,取海马组织,采用RT-PCR法测定Survivin mRNA和Caspase-3mRNA的表达.每组取5只大鼠,取海马组织,采用Western blot法测定Survivin蛋白和Caspase-3蛋白的表达.结果 七组间pH值、PaO2、PaCO2、HCO3-、BE和SaO2比较差异无统计学意义(P＞0.05).与C组比较,P1组、P2组、E1组和E2组海马Survivin和Caspase-3的mRNA和蛋白表达差异无统计学意义(P＞0.05),P3组和E3组海马Survivin mRNA和蛋白表达下调,Caspase-3 mRNA和蛋白表达上调(P＜0.05),海马CA1区神经元出现核固缩、染色质边集及凋亡小体.结论 较大剂量异丙酚和依托咪酯可能通过抑制Survivin的表达,增强Caspase-3的活性,导致幼龄大鼠海马神经元凋亡.
目的 評價異丙酚或依託咪酯對幼齡大鼠海馬神經元凋亡的影響.方法 雄性SD大鼠140隻,4週齡,體重40～60 g,採用隨機數字錶法,將大鼠隨機分為7組(n=20):對照組(C組)、異丙酚50 mg/kg組(P1組)、異丙酚100 mg/kg組(P2組)、異丙酚200 mg/kg組(P3組)、依託咪酯10 mg/kg組(E1組)、依託咪酯30 mg/kg組(E2組)和依託咪酯60 mg/kg組(E3組).P1組和E1組單次腹腔註射異丙酚50 mg/kg或依託咪酯10 mg/kg；P2組、P3組、E2組和E3組首次註射異丙酚50 mg/kg或依託咪酯10 mg/kg,待大鼠有體動反應後,再每次追加異丙酚50 mg/kg或依託咪酯10 mg/kg,直至給完總量.大鼠囌醒2h時,每組取5隻大鼠,抽取動脈血樣進行血氣分析.每組取5隻大鼠,取海馬CA1區,電鏡下觀察超微結構.每組取5隻大鼠,取海馬組織,採用RT-PCR法測定Survivin mRNA和Caspase-3mRNA的錶達.每組取5隻大鼠,取海馬組織,採用Western blot法測定Survivin蛋白和Caspase-3蛋白的錶達.結果 七組間pH值、PaO2、PaCO2、HCO3-、BE和SaO2比較差異無統計學意義(P＞0.05).與C組比較,P1組、P2組、E1組和E2組海馬Survivin和Caspase-3的mRNA和蛋白錶達差異無統計學意義(P＞0.05),P3組和E3組海馬Survivin mRNA和蛋白錶達下調,Caspase-3 mRNA和蛋白錶達上調(P＜0.05),海馬CA1區神經元齣現覈固縮、染色質邊集及凋亡小體.結論 較大劑量異丙酚和依託咪酯可能通過抑製Survivin的錶達,增彊Caspase-3的活性,導緻幼齡大鼠海馬神經元凋亡.
목적 평개이병분혹의탁미지대유령대서해마신경원조망적영향.방법 웅성SD대서140지,4주령,체중40～60 g,채용수궤수자표법,장대서수궤분위7조(n=20):대조조(C조)、이병분50 mg/kg조(P1조)、이병분100 mg/kg조(P2조)、이병분200 mg/kg조(P3조)、의탁미지10 mg/kg조(E1조)、의탁미지30 mg/kg조(E2조)화의탁미지60 mg/kg조(E3조).P1조화E1조단차복강주사이병분50 mg/kg혹의탁미지10 mg/kg；P2조、P3조、E2조화E3조수차주사이병분50 mg/kg혹의탁미지10 mg/kg,대대서유체동반응후,재매차추가이병분50 mg/kg혹의탁미지10 mg/kg,직지급완총량.대서소성2h시,매조취5지대서,추취동맥혈양진행혈기분석.매조취5지대서,취해마CA1구,전경하관찰초미결구.매조취5지대서,취해마조직,채용RT-PCR법측정Survivin mRNA화Caspase-3mRNA적표체.매조취5지대서,취해마조직,채용Western blot법측정Survivin단백화Caspase-3단백적표체.결과 칠조간pH치、PaO2、PaCO2、HCO3-、BE화SaO2비교차이무통계학의의(P＞0.05).여C조비교,P1조、P2조、E1조화E2조해마Survivin화Caspase-3적mRNA화단백표체차이무통계학의의(P＞0.05),P3조화E3조해마Survivin mRNA화단백표체하조,Caspase-3 mRNA화단백표체상조(P＜0.05),해마CA1구신경원출현핵고축、염색질변집급조망소체.결론 교대제량이병분화의탁미지가능통과억제Survivin적표체,증강Caspase-3적활성,도치유령대서해마신경원조망.
Objective To investigate the effects of propofol and etomidate on apoptosis in hippocampal neurons in rats.Methods One hundred and forty male 4 weeks old SD rats were randomly divided into 7 groups (n =20 each):control group (group C) ; groups P1,2,3 received intraperitoneal (IP) propofol 50,100 and 200mg/kg and groups E1,2,3 received IP etomidate 10,30 and 60 mg/kg respectively.Arterial blood samples were obtained at 2 h after the animals were fully awake for blood gas analysis.The animals were then sacrificed and their brains removed for microscopic examination of the ultrastructure of neurons in hippocampal CA1 area and detection of Survivin and Caspase-3 mRNA and protein expression in hippocampus by RT-PCR and Western blot analysis.Results There was no significant difference in PaO2,PaCO2,SaO2,HCO3-,BE and pH value among the 7 groups.The neurons in CA1 area were basically normal in groups C,P1 and E1 while condensation of the chromatin of the nucleus and apoptotic bodies were observed in groups P3 and E3.Caspase-3 mRNA and protein expression was significantly up-regulated while Survivin mRNA and protein down-regulated in groups P3 and E3.Conclusion High dose of propofol and etomidate may induce apoptosis in hippocampal neurons in rats by up-regulation of Caspase-3 expression and down-regulation of Survivin expression.
