CAJ | 학술논문

目的研究急性肝衰竭大鼠肝脏解偶联蛋白(UCP)2的表达及其与内毒索、氧自由基损伤的关系,进一步探讨肝衰竭的发病机制.方法将SD大鼠35只随机分为对照组(15只)、肝衰竭组(20只).腹腔注射硫代乙酰胺(TAA)制备肝衰竭大鼠模型.测定各组大鼠血清内毒素(ET)水平,肝脏组织UCP2 mRNA的表达、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,免疫组织化学观察UCP2表达.组间比较采用Mann-Whitney U检验或单因素方差分析.结果肝脏内UCP2 mRNA、MDA高于对照组[分别为相对表达量0.41±0.04对比0.31±0.04和(6.69±1.15)nmol/mg对比(1.73±0.18)nmol/mg,P＜0.01]; ET水平高于对照组[(15.63±4.90)×10-3EU/ml对比(8.07±2.26)×10-3EU/ml,P＜0.01]; SOD含量低于对照组[(98.56±17.75)U/mg对比(246.87±51.27)U/mg,P＜0.01]; UCP2 mRNA的表达与ET、SOD、MDA有一定的相关性(r值分别为0.952,-0.667,0.634,P＜0.01或P＜0.05);肝衰竭组UCP2表达增加.结论肝衰竭组大鼠肝脏内UCP2表达增加,UCP2 mRNA与ET、SOD、MDA水平有一定的相关性.
목적 연구급성간쇠갈대서간장해우련단백(UCP)2적표체급기여내독색、양자유기손상적관계,진일보탐토간쇠갈적발병궤제.방법장SD대서35지수궤분위대조조(15지)、간쇠갈조(20지).복강주사류대을선알(TAA)제비간쇠갈대서모형.측정각조대서혈청내독소(ET)수평,간장조직UCP2 mRNA적표체、병이철(MDA)함량、초양화물기화매(SOD)활성,면역조직화학관찰UCP2표체.조간비교채용Mann-Whitney U검험혹단인소방차분석.결과간장내UCP2 mRNA、MDA고우대조조[분별위상대표체량0.41±0.04대비0.31±0.04화(6.69±1.15)nmol/mg대비(1.73±0.18)nmol/mg,P＜0.01]; ET수평고우대조조[(15.63±4.90)×10-3EU/ml대비(8.07±2.26)×10-3EU/ml,P＜0.01]; SOD함량저우대조조[(98.56±17.75)U/mg대비(246.87±51.27)U/mg,P＜0.01]; UCP2 mRNA적표체여ET、SOD、MDA유일정적상관성(r치분별위0.952,-0.667,0.634,P＜0.01혹P＜0.05);간쇠갈조UCP2표체증가.결론 간쇠갈조대서간장내UCP2표체증가,UCP2 mRNA여ET、SOD、MDA수평유일정적상관성.
Objective To investigate the relationship between uncoupling protein 2 (UCP2) expression and the damage caused by oxygen free radicals in acute liver failure rat models. Methods Thirty-five male Sprague-Dawley rats were randomly divided into two groups: the control group (15 rats) and liver failure group (20 rats). The rats were injected intraperitoneally with thioacetamide (TAA) to induce models of acute liver failure. The levels of endotoxin (ET) were detected by double antibody sandwich enzyme-linked imrnunosorbent assay. The expression of liver UCP2 mRNA was detected by reverse transcription polymerase chain reactin. The superoxide dismutase (SOD) and malonaldehyde (MDA) were detected by spectrophotometry. The expression of UCP2 protein was observed by immunohistochemistry. The data of the two groups were compared using Mann-Whitney U test or ANOVA. Results The expression of UCP2 mRNA in liver failure group was higher as compared to the control group (P ＜ 0.01); the level of MDA and endotoxin of liver failure group were higher than that of the control group (P ＜ 0.01). SOD of the liver failure group was lower (P ＜ 0.01). There was a certain correlation between UCP2 mRNA expression and ET, SOD and MDA (r = 0.952, -0.667, 0. 634 respectively, P ＜ 0.05 or 0.01). Conclusions UCP2 is highly expressed in the livers of liver failure rats. A certain correlation perhaps existed between the expression of UCP2 mRNA and the seral SOD, MDA and ET.