中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2011年
9期
587-590
,共4页
沈方程%张红杰%王亚民%屠惠明%杨晓钟%邰雅宏%施瑞华
瀋方程%張紅傑%王亞民%屠惠明%楊曉鐘%邰雅宏%施瑞華
침방정%장홍걸%왕아민%도혜명%양효종%태아굉%시서화
白细胞介素-23受体,人%多态现象,遗传%炎症性肠病%点突变%年龄因素
白細胞介素-23受體,人%多態現象,遺傳%炎癥性腸病%點突變%年齡因素
백세포개소-23수체,인%다태현상,유전%염증성장병%점돌변%년령인소
Interleukin-23 receptor,human%Polymorphism,genetic%Inflammatory bowel diseases%Point mutation%Agefactors
目的 研究中国江苏汉族人群炎症性肠病(IBD)发病易感性和白细胞介素-23受体(IL-23R) rs1004819、rs1495965、rs1884444、rs2201841、rs6677188、rs7517847、rs7530511、rs10489629、rs10889677、rs11209026位点单核苷酸多态性(SNP)间的关系。方法 采用多重SNaPshot SNP分型技术，检测134名健康志愿者、135例溃疡性结肠炎(UC)和43例克罗恩病(CD)患者的基因多态性。实验数据使用SPSS 17.0软件进行分析。结果 UC组rs7530511位点CC基因型频率、CT基因型频率、C等位基因频率、T等位基因频率分别为99.26％(134/135)、0.74％(1/135)、99.63％(269/270)、0.37％(1/270)，正常对照组则分别为94.03％(126/134)、5.97％(8/134)、97.01％(260/268)、2.99％(8/268)，两组基因型频率比较P＝0.040、OR＝0.118、95％CI:0.014～0.953,两组等位基因频率比较P＝0.043、OR＝0.121、95％CI:0.015～0.973。在rs6677188野生型和突变型的UC患者中，各年龄段的分布比例不同，突变型者中青年患者较多，野生型者中中年患者较多，P值分别＝0.032和0.001。UC患者中rs6677188 AT基因型者多处于内镜缓解期(P＝0.032)。结论 IL-23R rs7530511位点突变是UC的保护性因素。rs6677188多态性与患者年龄和内镜下缓解与否有关。
目的 研究中國江囌漢族人群炎癥性腸病(IBD)髮病易感性和白細胞介素-23受體(IL-23R) rs1004819、rs1495965、rs1884444、rs2201841、rs6677188、rs7517847、rs7530511、rs10489629、rs10889677、rs11209026位點單覈苷痠多態性(SNP)間的關繫。方法 採用多重SNaPshot SNP分型技術，檢測134名健康誌願者、135例潰瘍性結腸炎(UC)和43例剋囉恩病(CD)患者的基因多態性。實驗數據使用SPSS 17.0軟件進行分析。結果 UC組rs7530511位點CC基因型頻率、CT基因型頻率、C等位基因頻率、T等位基因頻率分彆為99.26％(134/135)、0.74％(1/135)、99.63％(269/270)、0.37％(1/270)，正常對照組則分彆為94.03％(126/134)、5.97％(8/134)、97.01％(260/268)、2.99％(8/268)，兩組基因型頻率比較P＝0.040、OR＝0.118、95％CI:0.014～0.953,兩組等位基因頻率比較P＝0.043、OR＝0.121、95％CI:0.015～0.973。在rs6677188野生型和突變型的UC患者中，各年齡段的分佈比例不同，突變型者中青年患者較多，野生型者中中年患者較多，P值分彆＝0.032和0.001。UC患者中rs6677188 AT基因型者多處于內鏡緩解期(P＝0.032)。結論 IL-23R rs7530511位點突變是UC的保護性因素。rs6677188多態性與患者年齡和內鏡下緩解與否有關。
목적 연구중국강소한족인군염증성장병(IBD)발병역감성화백세포개소-23수체(IL-23R) rs1004819、rs1495965、rs1884444、rs2201841、rs6677188、rs7517847、rs7530511、rs10489629、rs10889677、rs11209026위점단핵감산다태성(SNP)간적관계。방법 채용다중SNaPshot SNP분형기술，검측134명건강지원자、135례궤양성결장염(UC)화43례극라은병(CD)환자적기인다태성。실험수거사용SPSS 17.0연건진행분석。결과 UC조rs7530511위점CC기인형빈솔、CT기인형빈솔、C등위기인빈솔、T등위기인빈솔분별위99.26％(134/135)、0.74％(1/135)、99.63％(269/270)、0.37％(1/270)，정상대조조칙분별위94.03％(126/134)、5.97％(8/134)、97.01％(260/268)、2.99％(8/268)，량조기인형빈솔비교P＝0.040、OR＝0.118、95％CI:0.014～0.953,량조등위기인빈솔비교P＝0.043、OR＝0.121、95％CI:0.015～0.973。재rs6677188야생형화돌변형적UC환자중，각년령단적분포비례불동，돌변형자중청년환자교다，야생형자중중년환자교다，P치분별＝0.032화0.001。UC환자중rs6677188 AT기인형자다처우내경완해기(P＝0.032)。결론 IL-23R rs7530511위점돌변시UC적보호성인소。rs6677188다태성여환자년령화내경하완해여부유관。
Objective To investigate the correlation between single nucleotide polymorphisms (SNP) of gene interleukin-23 receptor (IL-23R) rs1004819, rs1495965, rs1884444, rs2201841,rs6677188, rs7517847, rs7530511, rs10489629, rs10889677 and rs11209026 with susceptibility of inflammatory bowel disease (IBD) in Han population of Jiangsu province in China. Methods The gene polymorphism in 134 healthy volunteers, 135 cases of ulcerative colitis(UC) and 43 cases of Crohn's disease(CD) were detected with SNaPshot. Experimental data were analyzed with SPSS 17.0 software. Results In UC, genotype frequency of CC and CT on rs7530511 was 99.26％ (134/135)and 0.74％(1/135), allele frequency of C and T was 99.63％(269/270)and 0. 37％(1/270). While in normal controls, which were 94.03％(126/134), 5.97％(8/134), 97.01 ％(260/268)and 2.99％(8/268)respectively. Compared genotype frequency of these two group, P value was 0. 040 (OR＝0.118、95％CI:0.014～0.953). Compared allele frequency of these two group, P value was 0. 043 (OR＝0.121、95％CI:0.015～0.973). In wild type and mutation type UC patients, the age distribution was different, more young patients in mutation type while more middle-aged patients in wild type, P value was 0.032 and 0.001 respectively. Most UC patients of rs6677188 AT type were in remission under endoscope (P＝0.032). Conclusion The mutation of IL-23R rs7530511 may be a protective factor of UC. The polymorphism of rs6677188 was associated with the age of patients and the remission under endoscope.
