CAJ | 학술논문

目的探讨半乳糖凝集素3 mRNA(Galectin-3)和细胞分裂周期蛋白25B mRNA(cell division cycle 25B,CDC25B)在胃癌中的表达及其与临床病理参数及预后的关系.方法应用组织微阵列技术和原位杂交法检测220例胃癌组织和31份正常胃黏膜组织中Galectin-3 mRNA和CDC25B mRNA 的表达情况.结果 220例胃癌中Galectin-3 mRNA和CDC25B mRNA的阳性表达率分别为58.6%、54.1%;Galectin-3 mRNA表达与肿瘤大小、TNM(tumor,lymph node,metastasis)分期、浸润深度、脉管侵犯、淋巴结转移及远处转移呈正相关;CDC25B mRNA表达也与肿瘤大小、TNM分期、脉管侵犯、淋巴结转移及远处转移呈正相关;Galectin-3 mRNA表达与CDC25B mRNA表达呈正相关;Galectim3 mRNA和CDC25B mRNA阳性表达病例的平均生存时间和5年生存率均明显低于阴性表达的病例.结论 Galectin-3 和CDC25B基因的表达促进了胃癌的发生和发展,是指导临床治疗及评估预后的有效指标.
목적 탐토반유당응집소3 mRNA(Galectin-3)화세포분렬주기단백25B mRNA(cell division cycle 25B,CDC25B)재위암중적표체급기여림상병리삼수급예후적관계.방법 응용조직미진렬기술화원위잡교법검측220례위암조직화31빈정상위점막조직중Galectin-3 mRNA화CDC25B mRNA 적표체정황.결과 220례위암중Galectin-3 mRNA화CDC25B mRNA적양성표체솔분별위58.6%、54.1%;Galectin-3 mRNA표체여종류대소、TNM(tumor,lymph node,metastasis)분기、침윤심도、맥관침범、림파결전이급원처전이정정상관;CDC25B mRNA표체야여종류대소、TNM분기、맥관침범、림파결전이급원처전이정정상관;Galectin-3 mRNA표체여CDC25B mRNA표체정정상관;Galectim3 mRNA화CDC25B mRNA양성표체병례적평균생존시간화5년생존솔균명현저우음성표체적병례.결론 Galectin-3 화CDC25B기인적표체촉진료위암적발생화발전,시지도림상치료급평고예후적유효지표.
Objective To study the expression of Galectin-3 and CDC25B mRNA in gastric carcinoma and their correlation with clinical-pathological features and the survival time. Methods Tissue microarray (TMA) technique and in situ hybridization were used to detect the expression of Galectin-3 and CDC25B mRNA in 220 gastric carcinoma specimens and 31 normal gastric mucosa samples. Results In situ hybridization results revealed that from the 220 cases, the positive expression rate of Galectin-3 and CDC25B mRNA were 58. 6% and 54.1%, respectively. There was significant relationship between the Galectin-3 mRNA expression and tumor diameter, advanced TNM stage, invasion depth, vessel invasion, lymph node and distant metastasis. There was significant relationship between CDC25B mRNA expression and tumor diameter, advanced TNM stage, vessel invasion, lymph node and distant metastasis. In addition, there was a positive relationship of Galectin-3 and CDC25B mRNA expression. Finally, the mean survival time in cases with Galectin-3 and CDC25B mRNA positive expression was significantly shorter than those without Galectin-3 and CDC25B expression. Conclusion The expression of Galeetin-3 and CDC25B mRNA appears to act as a promoting factor in the onset and development of gastric cancer. It can be used as a marker of prognosis of gastric carcinoma in clinical practice.