CAJ | 학술논문

目的构建一种简便、可靠、直观的毛囊发育模型以检测毛囊细胞的毛发诱导能力,探讨毛囊形态发生和周期循环的分子机制.方法于裸鼠背部植入一开放小室,将新生C57BL/6鼠皮肤中的真皮和表皮细胞分离,并按一定的比例混合移植至小室内,1周后拆除小室,观察毛发形成及毛发拔除后的再生情况.结果细胞移植后1周,创面湿润无明显收缩,中间有淡红色半透明组织形成.移植后2周,创面完全愈合.移植后3周有黑色毛发长出皮肤.移植后4周,浓密黑色毛发垂直于皮肤表面生长,石蜡切片HE染色见毛囊结构发育完整.毛发拔除后1周能够再生出新的毛发.细胞以不同的比例移植,真皮细胞数量为1 × 107、表皮细胞数量降至1 ×106时,毛囊重构的效率并无明显改变.表皮细胞数量为1×107、真皮细胞数量降至5×106或者更少时,再生毛发的数量明显减少,两者单独移植均无毛发生长.结论新生鼠皮肤细胞以小室法移植后可以构建毛囊发育的完整模型,并可用于检测毛囊细胞的诱导能力,以研究毛囊形态的发生和周期循环的分子机制.
목적 구건일충간편、가고、직관적모낭발육모형이검측모낭세포적모발유도능력,탐토모낭형태발생화주기순배적분자궤제.방법 우라서배부식입일개방소실,장신생C57BL/6서피부중적진피화표피세포분리,병안일정적비례혼합이식지소실내,1주후탁제소실,관찰모발형성급모발발제후적재생정황.결과 세포이식후1주,창면습윤무명현수축,중간유담홍색반투명조직형성.이식후2주,창면완전유합.이식후3주유흑색모발장출피부.이식후4주,농밀흑색모발수직우피부표면생장,석사절편HE염색견모낭결구발육완정.모발발제후1주능구재생출신적모발.세포이불동적비례이식,진피세포수량위1 × 107、표피세포수량강지1 ×106시,모낭중구적효솔병무명현개변.표피세포수량위1×107、진피세포수량강지5×106혹자경소시,재생모발적수량명현감소,량자단독이식균무모발생장.결론 신생서피부세포이소실법이식후가이구건모낭발육적완정모형,병가용우검측모낭세포적유도능력,이연구모낭형태적발생화주기순배적분자궤제.
Objective To construct a convenient,reliable and visual model of hair follicle development to test the hair-inductive potential of follicular cells and investigate the molecular mechanism regulating hair follicle morphogenesis and cycling.Methods An open chamber was transplanted into the nude mice dorsal skin,dermal and epidermal cells isolated from newborn C57BL/6 mice skin were mixed at a specific ratio and then injected into the chamber together,1 week after transplantation,the chamber was removed,and then,hair formation and regeneration after hair plucking was observed.Results 1 week after cells implantation,the wound was moist without apparent contraction and among that pink and translucent tissue was formed.2 weeks after implantation,the wound healed completely.3 weeks after implantation,black hair grew from the skin was observed.4 weeks after implantation,thick and black hair grew from the skin vertically.Completely developed structure of hair follicle was observed with paraffin section and HE staining.1 week after plucking,new hair had regrown.The ratio of cell component was varied,whereas the other component was fixed at 1 × 107 cells.When the number of epidermal cells was reduced to 1 × 106 cells,the efficiency of hair follicle reconstitution was mostly unchanged.On the other hand,the density of newly formed hair was diminished considerably by reducing the number of dermal cells to 5 × 106 cells or lower.Neither epidermal cells nor dermal cells transplanted alone formed hair follicle.Conclusions Newborn mice skin cells transplanted by chamber method can construct a complete model of hair follicle development,which can be used to test the hair-inductive potential of follicular cells and investigate the molecular mechanism regulating hair follicle morphogenesis and cycling.