CAJ | 학술논문

[目的]建立"丰香"草莓的高效脱毒及快速繁殖体系.[方法]以"丰香"草莓的茎尖为外植体,高温处理和1‰氯化汞消毒后进行丛生芽诱导和生根培养,研究不同激素配比对出芽率和生根率的影响,并对脱毒苗的病毒感染情况进行检测.[结果]6-BA 0.5 mg/L+NAA 0.01 mg/L培养基上芽分化率最高,有效苗达98%,将丛生芽分成单株,用相同的培养基进行继代培养,可获得大量繁殖苗;用培养基1/2 MS+0.02 mg/LNAA对小苗进行培养,小苗15 d 即可生根,20后根系发达,平均每苗可产生3～6条根;病毒检测结果显示,脱毒苗的脱毒率达到100%.[结论]"丰香"草莓的最佳芽诱导培养基为6-BA 0.5 mg/L+NAA 0.01 mg/L,最佳生根培养基为1/2 MS+0.02 mg/L NAA.
[목적]건립"봉향"초매적고효탈독급쾌속번식체계.[방법]이"봉향"초매적경첨위외식체,고온처리화1‰록화홍소독후진행총생아유도화생근배양,연구불동격소배비대출아솔화생근솔적영향,병대탈독묘적병독감염정황진행검측.[결과]6-BA 0.5 mg/L+NAA 0.01 mg/L배양기상아분화솔최고,유효묘체98%,장총생아분성단주,용상동적배양기진행계대배양,가획득대량번식묘;용배양기1/2 MS+0.02 mg/LNAA대소묘진행배양,소묘15 d 즉가생근,20후근계발체,평균매묘가산생3～6조근;병독검측결과현시,탈독묘적탈독솔체도100%.[결론]"봉향"초매적최가아유도배양기위6-BA 0.5 mg/L+NAA 0.01 mg/L,최가생근배양기위1/2 MS+0.02 mg/L NAA.
[Objective] The study was to establish the system of efficient detoxification and rapid propagation for "Fengxiang" strawberry. [Method] With shoot tips of "Fengxiang" strawberry as the explants, they were treated with high temperature and disinfectd by 1‰ HgCl_2, and then multiple shoots were induced and rooting culture was carried out. The effects of different hormone combinations on rates of budding and rooting were studied, and the infection status of virus-free seedlings on virus was detected. [Result] Bud differentiation rate of explants on medium of 6-BA 0.5 mg/L+NAA 0.01 mg/L was the highest with effective buds of 98%. Plenty of propagation seedlings could be obtained when single plant from multiple shoots was cultured with the same medium. The young seedlings could root after 15 d of culture on medium of 1/2 MS+0.02 mg/L NAA, and their roots was developed after 20 d of culture with 3-6 roots per seedling. The results of virus detection showed that the virus elimination rate of virus-free seedlings reached 100%. [Conclusion] The optimum bud induction and rooting mediums for "Fengxiang" strawberry were 6-BA 0.5 mg/L+NAA 0.01 mg/L and 1/2 MS+0.02 mg/LNAA resp..